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Literature DB >> 206266

Problems encountered in measuring the activity of phosphatidate phosphohydrolase.

Abstract

The measurement of phosphate release from phosphatidate overestimates the microsomal activity of phosphatidate phosphohydrolase from rat liver, since phosphate is also produced via the glycerol phosphate that results from the deacylation of phosphatidate. The determination of phosphate production can be a reliable assay for the soluble phosphatidate phosphohydrolase in rat liver, because the glycerol phosphate formed is not hydrolysed under the conditions used.

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Year: 1978 PMID： 206266 PMCID： PMC1184155 DOI： 10.1042/bj1710263

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

22 in total

1. A rapid method of total lipid extraction and purification.

Authors: E G BLIGH; W J DYER
Journal: Can J Biochem Physiol Date: 1959-08

2. Metabolism of phospholipids. V. Studies of phosphatidic acid phosphatase.

Authors: R COLEMAN; G HUEBSCHER
Journal: Biochim Biophys Acta Date: 1962-01-29

3. Stimulation of hepatic phosphatidate phosphohydrolase activity by a single dose of ethanol;.

Authors: M J Savolainen
Journal: Biochem Biophys Res Commun Date: 1977-03-21 Impact factor: 3.575

4. Phosphatidate phosphohydrolase and the regulation of glycerolipid biosynthesis.

Authors: H J Fallon; R G Lamb; S C Jamdar
Journal: Biochem Soc Trans Date: 1977 Impact factor: 5.407

5. The effects of amphiphilic cationic drugs and inorganic cations on the activity of phosphatidate phosphohydrolase.

Authors: M Bowley; J Cooling; S L Burditt; D N Brindley
Journal: Biochem J Date: 1977-09-01 Impact factor: 3.857

6. The effect of chronic diabetes, induced by streptozotocin, on the activities of some enzymes of glycerolipid synthesis in rat liver.

Authors: P H Whiting; M Bowley; R G Sturton; P H Pritchard; D N Brindley; J N Hawthorne
Journal: Biochem J Date: 1977-11-15 Impact factor: 3.857

7. Some aspects of the physiological and pharmacological control of the synthesis of triacylglycerols and phospholipids.

Authors: D N Brindley
Journal: Int J Obes Date: 1978

8. The effects of acute ethanol feeding and of chronic benfluorex administration on the activities of some enzymes of glycerolipid synthesis in rat liver and adipose tissue.

Authors: P H Pritchard; M Bowley; S L Burditt; J Cooling; H P Glenny; N Lawson; R G Sturton; D N Brindley
Journal: Biochem J Date: 1977-09-15 Impact factor: 3.857

9. Factors controlling the activities of phosphatidate phosphohydrolase and phosphatidate cytidylyltransferase. The effects of chlorpromazine, demethylimipramine, cinchocaine, norfenfluramine, mepyramine and magnesium ions.

Authors: R G Sturton; D N Brindley
Journal: Biochem J Date: 1977-01-15 Impact factor: 3.857

10. The nature of the stimulatory role of the supernatant fraction on triglyceride synthesis by the alpha-Glycerophosphate pathway.

Authors: J M Johnston; G A Rao; P A Lowe; B E Schwarz
Journal: Lipids Date: 1967-01 Impact factor: 1.880

22 in total

1. The involvement of glucocorticoids in regulating the activity of phosphatidate phosphohydrolase and the synthesis of triacylglycerols in the liver. Effects of feeding rats with glucose, sorbitol, fructose, glycerol and ethanol.

Authors: D N Brindley; J Cooling; S L Burditt; P H Pritchard; S Pawson; R G Sturton
Journal: Biochem J Date: 1979-04-15 Impact factor: 3.857

2. A rapid assay for measuring the activity and the Mg2+ and Ca2+ requirements of phosphatidate phosphohydrolase in cytosolic and microsomal fractions of rat liver.

Authors: A Martin; P Hales; D N Brindley
Journal: Biochem J Date: 1987-07-15 Impact factor: 3.857

3. The use of two populations of hepatocytes with different triacylglycerol contents as a model to study the accumulation of liver lipid in the laying hen.

Authors: K E Cross; P F Dodds
Journal: Biochem J Date: 1988-10-01 Impact factor: 3.857

4. The effects of cortisol, corticotropin and thyroxine on the synthesis of glycerolipids and on the phosphatidate phosphohydrolase activity in rat liver.

Authors: H P Glenny; D N Brindley
Journal: Biochem J Date: 1978-12-15 Impact factor: 3.857

5. Mechanisms for the effects of ethanol on hepatic phosphatidate phosphohydrolase.

Authors: M J Savolainen; I E Hassinen
Journal: Biochem J Date: 1978-12-15 Impact factor: 3.857

6. The involvement of phosphatidate phosphohydrolase and phospholipase A activities in the control of hepatic glycerolipid synthesis. Effects of acute feeding with glucose, fructose, sorbitol, glycerol and ethanol.

Authors: R G Sturton; P H Pritchard; L Y Han; D N Brindley
Journal: Biochem J Date: 1978-08-15 Impact factor: 3.857

7. Effects of chronic modification of dietary fat and carbohydrate on the insulin, corticosterone and metabolic responses of rats fed acutely with glucose, fructose or ethanol.

Authors: D N Brindley; J Cooling; H P Glenny; S L Burditt; I S McKechnie
Journal: Biochem J Date: 1981-11-15 Impact factor: 3.857

8. Propranolol-induced inhibition of rat brain cytoplasmic phosphatidate phosphohydrolase.

Authors: A S Pappu; G Hauser
Journal: Neurochem Res Date: 1983-12 Impact factor: 3.996

9. Partial purification and characterization of the soluble phosphatidate phosphohydrolase of rat liver.

Authors: S C Butterwith; R Hopewell; D N Brindley
Journal: Biochem J Date: 1984-06-15 Impact factor: 3.857

10. The activities of lipoprotein lipase and of enzymes involved in triacylglycerol synthesis in rat adipose tissue. Effects of starvation, dietary modification and of corticotropin injection.

Authors: N Lawson; A D Pollard; R J Jennings; M I Gurr; D N Brindley
Journal: Biochem J Date: 1981-11-15 Impact factor: 3.857