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Literature DB >> 6087797

Partial purification and characterization of the soluble phosphatidate phosphohydrolase of rat liver.

S C Butterwith, R Hopewell, D N Brindley.

Abstract

A method is described by which the Mg2+-stimulated phosphatidate phosphohydrolase can be purified from the soluble fraction of liver from ethanol-treated rats. The increase in specific activity was about 416-fold. This involved purification by adsorption on calcium phosphate, chromatography on DE-52 DEAE-cellulose, separation on Ultrogel AcA-34 and chromatography on CM-Sepharose 6B. The effects of phosphatidylcholine, phosphatidate and Mg2+, Mn2+ and Zn2+ on the activity are described. Inhibitor studies indicate that the phosphohydrolase contains functional thiol groups and arginine residues.

Entities: Chemical Species

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Year: 1984 PMID： 6087797 PMCID： PMC1153702 DOI： 10.1042/bj2200825

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

30 in total

1. The involvement of glucocorticoids in regulating the activity of phosphatidate phosphohydrolase and the synthesis of triacylglycerols in the liver. Effects of feeding rats with glucose, sorbitol, fructose, glycerol and ethanol.

Authors: D N Brindley; J Cooling; S L Burditt; P H Pritchard; S Pawson; R G Sturton
Journal: Biochem J Date: 1979-04-15 Impact factor: 3.857

2. Hormonal regulation of hepatic soluble phosphatidate phosphohydrolase. Induction by cortisol in vivo and in isolated perfused rat liver.

Authors: M A Lehtonen; M J Savolainen; I E Hassinen
Journal: FEBS Lett Date: 1979-03-01 Impact factor: 4.124

3. The phosphohydrolase activity in lamellar bodies and its relationship to phosphatidylglycerol and lung surfactant formation.

Authors: J M Johnston; G Reynolds; M B Wylie; P C MacDonald
Journal: Biochim Biophys Acta Date: 1978-10-25

4. The effects of amphiphilic cationic drugs and inorganic cations on the activity of phosphatidate phosphohydrolase.

Authors: M Bowley; J Cooling; S L Burditt; D N Brindley
Journal: Biochem J Date: 1977-09-01 Impact factor: 3.857

5. Stimulation of the activities of phosphatidate phosphohydrolase and tyrosine aminotransferase in rat hepatocytes by glucocorticoids.

Authors: R J Jennings; N Lawson; R Fears; D N Brindley
Journal: FEBS Lett Date: 1981-10-12 Impact factor: 4.124

6. Separation and characterization of the membrane-bound and aqueously dispersed phosphatidate phosphatidic acid phosphohydrolase activities in rat lung.

Authors: P G Casola; F Possmayer
Journal: Biochim Biophys Acta Date: 1981-05-22

7. Effects of starvation, corticotropin injection and ethanol feeding on the activity and amount of phosphatidate phosphohydrolase in rat liver.

Authors: R G Sturton; S C Butterwith; S L Burditt; D N Brindley
Journal: FEBS Lett Date: 1981-04-20 Impact factor: 4.124

8. Factors controlling the metabolism of phosphatidate by phosphohydrolase and phospholipase A-type activities. Effects of magnesium, calcium and amphiphilic cationic drugs.

Authors: R G Sturton; D N Brindley
Journal: Biochim Biophys Acta Date: 1980-09-08

9. Factors controlling the activities of phosphatidate phosphohydrolase and phosphatidate cytidylyltransferase. The effects of chlorpromazine, demethylimipramine, cinchocaine, norfenfluramine, mepyramine and magnesium ions.

Authors: R G Sturton; D N Brindley
Journal: Biochem J Date: 1977-01-15 Impact factor: 3.857

10. Pulmonary phosphatidic acid phosphohydrolase: further studies on the activities in rat lung responsible for the hydrolysis of membrane-bound and aqueously dispersed phosphatidate.

Authors: P G Casola; F Possmayer
Journal: Can J Biochem Date: 1981-07

14 in total

1. Lipin proteins form homo- and hetero-oligomers.

Authors: Guang-Hui Liu; Jing Qu; Anne E Carmack; Hyun Bae Kim; Chang Chen; Hongmei Ren; Andrew J Morris; Brian N Finck; Thurl E Harris
Journal: Biochem J Date: 2010-11-15 Impact factor: 3.857

2. A rapid assay for measuring the activity and the Mg2+ and Ca2+ requirements of phosphatidate phosphohydrolase in cytosolic and microsomal fractions of rat liver.

Authors: A Martin; P Hales; D N Brindley
Journal: Biochem J Date: 1987-07-15 Impact factor: 3.857

Review 3. A review of phosphatidate phosphatase assays.

Authors: Prabuddha Dey; Gil-Soo Han; George M Carman
Journal: J Lipid Res Date: 2020-09-22 Impact factor: 5.922

4. Properties of phosphatidate phosphohydrolase in rat adipose tissue.

Authors: S C Jamdar; W F Cao
Journal: Biochem J Date: 1994-08-01 Impact factor: 3.857

5. Adipose-tissue Mg2+-dependent phosphatidate phosphohydrolase. Control of activity and subcellular distribution in vitro and in vivo.

Authors: S J Taylor; E D Saggerson
Journal: Biochem J Date: 1986-10-15 Impact factor: 3.857

6. Characterization of the human LPIN1-encoded phosphatidate phosphatase isoforms.

Authors: Gil-Soo Han; George M Carman
Journal: J Biol Chem Date: 2010-03-15 Impact factor: 5.157

7. Regulation of the translocation of phosphatidate phosphohydrolase between the cytosol and the endoplasmic reticulum of rat liver. Effects of unsaturated fatty acids, spermine, nucleotides, albumin and chlorpromazine.

Authors: R Hopewell; P Martin-Sanz; A Martin; J Saxton; D N Brindley
Journal: Biochem J Date: 1985-12-01 Impact factor: 3.857

Review 8. Phosphatidate phosphatases of mammals, yeast, and higher plants.

Authors: M G Kocsis; R J Weselake
Journal: Lipids Date: 1996-08 Impact factor: 1.880

9. Microsomal phosphatidate phosphatase in maturing safflower seeds.

Authors: K Ichihara; S Norikura; S Fujii
Journal: Plant Physiol Date: 1989-06 Impact factor: 8.340

10. Can phosphorylation of phosphatidate phosphohydrolase by a cyclic AMP-dependent mechanism regulate its activity and subcellular distribution and control hepatic glycerolipid synthesis?

Authors: S C Butterwith; A Martin; D N Brindley
Journal: Biochem J Date: 1984-09-01 Impact factor: 3.857