Literature DB >> 2062640

In situ hybridization with fluoresceinated DNA.

J Wiegant1, T Ried, P M Nederlof, M van der Ploeg, H J Tanke, A K Raap.   

Abstract

We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection sensitivity of microscopic imaging of in situ hybridized, fluoresceinated DNA with an integrating solid state camera for rapid cosmid mapping is illustrated. Combination of fluoresceinated DNA with biotinated and digoxigeninated DNAs allowed easy performance of triple fluorescence in situ hybridization. The potential of these techniques for DNA mapping, cytogenetics and biological dosimetry is briefly discussed.

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Year:  1991        PMID： 2062640      PMCID： PMC328316          DOI： 10.1093/nar/19.12.3237

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

1.  Interphase cytogenetics of brain tumors.

Authors:  E P Arnoldus; I A Noordermeer; A C Peters; J H Voormolen; G T Bots; A K Raap; M van der Ploeg
Journal:  Genes Chromosomes Cancer       Date:  1991-03       Impact factor: 5.006

2.  A non-radioactive in situ hybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands.

Authors:  A H Hopman; J Wiegant; G I Tesser; P Van Duijn
Journal:  Nucleic Acids Res       Date:  1986-08-26       Impact factor: 16.971

3.  Molecular analysis of a deletion polymorphism in alpha satellite of human chromosome 17: evidence for homologous unequal crossing-over and subsequent fixation.

Authors:  J S Waye; H F Willard
Journal:  Nucleic Acids Res       Date:  1986-09-11       Impact factor: 16.971

4.  Bi-color detection of two target DNAs by non-radioactive in situ hybridization.

Authors:  A H Hopman; J Wiegant; A K Raap; J E Landegent; M van der Ploeg; P van Duijn
Journal:  Histochemistry       Date:  1986

5.  Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen.

Authors:  J Bubeník; M Baresová; V Viklický; J Jakoubková; H Sainerová; J Donner
Journal:  Int J Cancer       Date:  1973-05       Impact factor: 7.396

6.  A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochromelabelled RNA.

Authors:  J G Bauman; J Wiegant; P Borst; P van Duijn
Journal:  Exp Cell Res       Date:  1980-08       Impact factor: 3.905

7.  Cytochemical hybridisation with fluorochrome-labelled RNA. III. Increased sensitivity by the use of anti-fluorescein antibodies.

Authors:  J G Bauman; J Wiegant; P van Duijn
Journal:  Histochemistry       Date:  1981

8.  Characterization of a cloned DNA sequence that is present at centromeres of all human autosomes and the X chromosome and shows polymorphic variation.

Authors:  E W Jabs; S F Wolf; B R Migeon
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

9.  Cloning of human satellite III DNA: different components are on different chromosomes.

Authors:  H J Cooke; J Hindley
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

10.  Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization.

Authors:  J E Landegent; N Jansen in de Wal; G J van Ommen; F Baas; J J de Vijlder; P van Duijn; M Van der Ploeg
Journal:  Nature       Date:  1985 Sep 12-18       Impact factor: 49.962
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  27 in total

1.  Rapid detection of chromosome aneuploidies in uncultured amniocytes by using fluorescence in situ hybridization (FISH).

Authors:  K Klinger; G Landes; D Shook; R Harvey; L Lopez; P Locke; T Lerner; R Osathanondh; B Leverone; T Houseal
Journal:  Am J Hum Genet       Date:  1992-07       Impact factor: 11.025

2.  Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescence in situ hybridization (FISH).

Authors:  X B Zhong; J Hans de Jong; P Zabel
Journal:  Chromosome Res       Date:  1996-01       Impact factor: 5.239

3.  XR-C1, a new CHO cell mutant which is defective in DNA-PKcs, is impaired in both V(D)J coding and signal joint formation.

Authors:  A Errami; D M He; A A Friedl; W J Overkamp; B Morolli; E A Hendrickson; F Eckardt-Schupp; M Oshimura; P H Lohman; S P Jackson; M Z Zdzienicka
Journal:  Nucleic Acids Res       Date:  1998-07-01       Impact factor: 16.971

Review 4.  RNA molecules lighting up under the microscope.

Authors:  R W Dirks
Journal:  Histochem Cell Biol       Date:  1996-08       Impact factor: 4.304

Review 5.  Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.

Authors:  E J Speel; F C Ramaekers; A H Hopman
Journal:  Histochem J       Date:  1995-11

6.  Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family.

Authors:  María Ubeda-Manzanaro; Manuel A Merlo; José L Palazón; Ismael Cross; Carmen Sarasquete; Laureana Rebordinos
Journal:  Genetica       Date:  2010-05-04       Impact factor: 1.082

7.  Combined DNA-RNA fluorescent in situ hybridization (FISH) to study X chromosome inactivation in differentiated female mouse embryonic stem cells.

Authors:  Tahsin Stefan Barakat; Joost Gribnau
Journal:  J Vis Exp       Date:  2014-06-14       Impact factor: 1.355

8.  Detection of nondisjunction and recombination in meiotic and postmeiotic cells from XYSxr [XY,Tp(Y)1Ct] mice using multicolor fluorescence in situ hybridization.

Authors:  T Ashley; T Ried; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

9.  Human chromosome 11 complements ataxia-telangiectasia cells but does not complement the defect in AT-like Chinese hamster cell mutants.

Authors:  W Jongmans; J Wiegant; M Oshimura; M R James; P H Lohman; M Z Zdzienicka
Journal:  Hum Genet       Date:  1993-10-01       Impact factor: 4.132

10.  Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.

Authors:  Kah Teong Soh; Joseph D Tario; Sean Colligan; Orla Maguire; Dalin Pan; Hans Minderman; Paul K Wallace
Journal:  Curr Protoc Cytom       Date:  2016-01-06
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