| Literature DB >> 20614991 |
Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Y Berezin, Walter J Akers, Samuel Achilefu.
Abstract
While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.Mesh:
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Year: 2010 PMID: 20614991 PMCID: PMC2881927 DOI: 10.1117/1.3420209
Source DB: PubMed Journal: J Biomed Opt ISSN: 1083-3668 Impact factor: 3.170