PURPOSE: Dose response and repair kinetics of phosphorylated histone H2A isoform X (gamma-H2AX) foci in T-lymphocytes were investigated in the low-dose range after in vitro irradiation of whole blood and T-lymphocytes with 100 kVp X-rays and (60)Co gamma-rays. MATERIALS AND METHODS: Whole blood or isolated T-lymphocytes were irradiated in vitro and gamma-H2AX foci were scored. Dose response was determined in the 0-500 mGy dose range. Foci kinetics were studied at doses of 5 and 200 mGy up to 24 h post-irradiation. RESULTS: After X-irradiation, the dose response for whole blood shows a biphasic behaviour with a low-dose hypersensitivity, which is less pronounced for isolated T-lymphocytes. In contrast, gamma-radiation shows a linear dose response for both irradiation conditions. Concerning repair kinetics, delayed repair was found after X-ray whole blood irradiation (5 and 200 mGy) with 40% of the foci persisting 24 h post-irradiation. This number of foci is reduced to 10% after irradiation of isolated T-lymphocytes with 200 mGy X-rays. On the contrary, gamma-H2AX foci are reduced to background levels 24 h post-irradiation with 200 mGy (60)Co gamma-rays. CONCLUSION: gamma-H2AX foci response and repair kinetics depend on irradiation conditions and radiation quality, possibly linked to Bystander response.
PURPOSE: Dose response and repair kinetics of phosphorylated histone H2A isoform X (gamma-H2AX) foci in T-lymphocytes were investigated in the low-dose range after in vitro irradiation of whole blood and T-lymphocytes with 100 kVp X-rays and (60)Co gamma-rays. MATERIALS AND METHODS: Whole blood or isolated T-lymphocytes were irradiated in vitro and gamma-H2AX foci were scored. Dose response was determined in the 0-500 mGy dose range. Foci kinetics were studied at doses of 5 and 200 mGy up to 24 h post-irradiation. RESULTS: After X-irradiation, the dose response for whole blood shows a biphasic behaviour with a low-dose hypersensitivity, which is less pronounced for isolated T-lymphocytes. In contrast, gamma-radiation shows a linear dose response for both irradiation conditions. Concerning repair kinetics, delayed repair was found after X-ray whole blood irradiation (5 and 200 mGy) with 40% of the foci persisting 24 h post-irradiation. This number of foci is reduced to 10% after irradiation of isolated T-lymphocytes with 200 mGy X-rays. On the contrary, gamma-H2AX foci are reduced to background levels 24 h post-irradiation with 200 mGy (60)Co gamma-rays. CONCLUSION: gamma-H2AX foci response and repair kinetics depend on irradiation conditions and radiation quality, possibly linked to Bystander response.
Authors: Qi Wang; Younghyun Lee; Monica Pujol-Canadell; Jay R Perrier; Lubomir Smilenov; Andrew Harken; Guy Garty; David J Brenner; Brian Ponnaiya; Helen C Turner Journal: Cytogenet Genome Res Date: 2021-09-06 Impact factor: 1.941
Authors: Felix Zwicker; Benedict Swartman; Florian Sterzing; Gerald Major; Klaus-Josef Weber; Peter E Huber; Christian Thieke; Jürgen Debus; Klaus Herfarth Journal: Radiat Oncol Date: 2011-06-07 Impact factor: 3.481
Authors: Uta Eberlein; Carina Nowak; Christina Bluemel; Andreas Konrad Buck; Rudolf Alexander Werner; Harry Scherthan; Michael Lassmann Journal: Eur J Nucl Med Mol Imaging Date: 2015-06-06 Impact factor: 9.236