Literature DB >> 205977

A simple technique to assess the total number of Onchocerca volvulus microfilariae in skin snips.

H Schulz-Key.   

Abstract

A technique for the assessment of the total number of microfilariae in skin snips from onchocerciasis patients is described. For the digestion of the skin tissue the biopsies are incubated at room temperature for 24 hours in medium 199, containing 3 mg/ml collagenase and antibiotics. This process leaves the microfilariae unharmed. A comparative study of the emergence of the microfilariae in different media showed that only 20% of the total number were released after 30 minutes in distilled water. After 24 hours incubation in isotonic saline 80% were found.

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Year:  1978        PMID： 205977

Source DB:  PubMed          Journal:  Tropenmed Parasitol        ISSN： 0303-4208


  15 in total

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Authors:  Moses N Katabarwa; Tom Lakwo; Peace Habomugisha; Stella Agunyo; Edson Byamukama; David Oguttu; Ephraim Tukesiga; Dickson Unoba; Patrick Dramuke; Ambrose Onapa; Edridah M Tukahebwa; Dennis Lwamafa; Frank Walsh; Thomas R Unnasch
Journal:  Am J Trop Med Hyg       Date:  2013-05-20       Impact factor: 2.345

3.  Onchocerca raillieti: release from skin snips, maintenance in vitro and periodicity of microfilariae.

Authors:  H S Hussein; S E el Sammani
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4.  The disappearance of onchocerciasis without intervention in Tigray Region in Northwest Ethiopia.

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5.  Clinical trials of amodiaquine in onchocerciasis.

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6.  Sensitivity of a polymerase chain reaction-based assay to detect Onchocerca volvulus DNA in skin biopsies.

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10.  Fifteen years of annual mass treatment of onchocerciasis with ivermectin have not interrupted transmission in the west region of cameroon.

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