Literature DB >> 20591817

Dependence of beam broadening on detection angle in scanning transmission electron microtomography.

Sohei Motoki1, Takeshi Kaneko, Yoshitaka Aoyama, Hideo Nishioka, Yoshihiro Okura, Yukihito Kondo, Hiroshi Jinnai.   

Abstract

It has been shown that scanning transmission electron microtomography (STEMT) is quite effective for observing specimens with thicknesses on the order of micrometers in three dimensions (3D). In STEMT, the specimen is scanned using a focused electron beam, and the electrons from the convergence point are detected at the detector placed at a certain detection angle. Until recently, a wide detection angle corresponding to the mode often called the dark-field (DF) mode was mainly used. Although the detection angle can vary and is one of the crucial experimental factors in STEMT, its effect on 3D reconstruction has never been discussed from either an experimental or a theoretical viewpoint. Moreover, the effectiveness of another mode of electron tomography, transmission electron microtomography (TEMT), is not clear. In the present study, a polymeric specimen, an acrylonitrile butadiene styrene resin, with a thickness of ~1 mum and a fixed volume was observed using three different modes, namely, TEMT, small detection-angle STEMT referred to as bright-field STEMT, and DF-STEMT, in order to examine their advantages and disadvantages by observing multiple scattering of electrons inside the specimen.

Entities:  

Year:  2010        PMID: 20591817     DOI: 10.1093/jmicro/dfq030

Source DB:  PubMed          Journal:  J Electron Microsc (Tokyo)        ISSN: 0022-0744


  4 in total

1.  Dual-axis electron tomography of biological specimens: Extending the limits of specimen thickness with bright-field STEM imaging.

Authors:  Alioscka A Sousa; Afrouz A Azari; Guofeng Zhang; Richard D Leapman
Journal:  J Struct Biol       Date:  2010-11-03       Impact factor: 2.867

Review 2.  Development and application of STEM for the biological sciences.

Authors:  Alioscka A Sousa; Richard D Leapman
Journal:  Ultramicroscopy       Date:  2012-05-18       Impact factor: 2.689

3.  Deep learning-based noise filtering toward millisecond order imaging by using scanning transmission electron microscopy.

Authors:  Shiro Ihara; Hikaru Saito; Mizumo Yoshinaga; Lavakumar Avala; Mitsuhiro Murayama
Journal:  Sci Rep       Date:  2022-08-05       Impact factor: 4.996

4.  Electron tomography provides a direct link between the Payne effect and the inter-particle spacing of rubber composites.

Authors:  Lech Staniewicz; Thomas Vaudey; Christophe Degrandcourt; Marc Couty; Fabien Gaboriaud; Paul Midgley
Journal:  Sci Rep       Date:  2014-12-09       Impact factor: 4.379

  4 in total

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