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Literature DB >> 20588500

Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation.

Jeffrey J Field¹, Ramón Carriles, Kraig E Sheetz, Eric V Chandler, Erich E Hoover, Shane E Tillo, Thom E Hughes, Anne W Sylvester, David Kleinfeld, Jeff A Squier.

Abstract

A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores-rhodamine B, a red fluorescent protein, and CdSe quantum dots-while manipulating the phase of the laser excitation pulse at the focus. In all cases a transform-limited pulse maximized the total yield to insure the highest signal-to-noise ratio. Further, we find evidence of fluorescence antibleaching in quantum dot samples.

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Year: 2010 PMID： 20588500 PMCID： PMC4151303 DOI： 10.1364/OE.18.013661

Source DB: PubMed Journal: Opt Express ISSN： 1094-4087 Impact factor: 3.894

18 in total

Review 1. Deep tissue two-photon microscopy.

Authors: Fritjof Helmchen; Winfried Denk
Journal: Nat Methods Date: 2005-12 Impact factor: 28.547

2. Major signal increase in fluorescence microscopy through dark-state relaxation.

Authors: Gerald Donnert; Christian Eggeling; Stefan W Hell
Journal: Nat Methods Date: 2006-12-10 Impact factor: 28.547

3. Simultaneous visualization of spatial and chromatic aberrations by two-dimensional Fourier transform spectral interferometry.

Authors: W Amir; T A Planchon; C G Durfee; J A Squier; P Gabolde; R Trebino; M Müller
Journal: Opt Lett Date: 2006-10-01 Impact factor: 3.776

4. Quartic-phase-limited grism-based ultrashort pulse shaper.

Authors: Jeffrey J Field; Charles G Durfee; Jeff A Squier; Steve Kane
Journal: Opt Lett Date: 2007-11-01 Impact factor: 3.776

5. Simultaneous multifocal, multiphoton, photon counting microscopy.

Authors: Ramón Carriles; Kraig E Sheetz; Erich E Hoover; Jeff A Squier; Virginijus Barzda
Journal: Opt Express Date: 2008-07-07 Impact factor: 3.894

6. Effect of pulse shape on the efficiency of multiphoton processes: implications for biological microscopy.

Authors: C J Bardeen; V V Yakovlev; J A Squier; K R Wilson; S D Carpenter; P M Weber
Journal: J Biomed Opt Date: 1999-07 Impact factor: 3.170

7. Characterization of a High Efficiency, Ultrashort Pulse Shaper Incorporating a Reflective 4096-Element Spatial Light Modulator.

Authors: Jeffrey J Field; Thomas A Planchon; Wafa Amir; Charles G Durfee; Jeff A Squier
Journal: Opt Commun Date: 2007-10-15 Impact factor: 2.310

8. Dispersion pre-compensation of 15 femtosecond optical pulses for high-numerical-aperture objectives.

Authors:
Journal: J Microsc Date: 1998-08 Impact factor: 1.758

9. Two-photon laser scanning fluorescence microscopy.

Authors: W Denk; J H Strickler; W W Webb
Journal: Science Date: 1990-04-06 Impact factor: 47.728

10. Absolute two-photon absorption spectra and two-photon brightness of orange and red fluorescent proteins.

Authors: M Drobizhev; S Tillo; N S Makarov; T E Hughes; A Rebane
Journal: J Phys Chem B Date: 2009-01-29 Impact factor: 2.991

13 in total

1. Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy.

Authors: Jonathan D Driscoll; Andy Y Shih; Satish Iyengar; Jeffrey J Field; G Allen White; Jeffrey A Squier; Gert Cauwenberghs; David Kleinfeld
Journal: J Neurophysiol Date: 2011-04-06 Impact factor: 2.714

2. A pragmatic guide to multiphoton microscope design.

Authors: Michael D Young; Jeffrey J Field; Kraig E Sheetz; Randy A Bartels; Jeff Squier
Journal: Adv Opt Photonics Date: 2015-06-30 Impact factor: 20.107

3. Optimizing pulse compressibility in completely all-fibered Ytterbium chirped pulse amplifiers for in vivo two photon laser scanning microscopy.

Authors: A Fernández; L Grüner-Nielsen; M Andreana; M Stadler; S Kirchberger; C Sturtzel; M Distel; L Zhu; W Kautek; R Leitgeb; A Baltuska; K Jespersen; A Verhoef
Journal: Biomed Opt Express Date: 2017-07-03 Impact factor: 3.732

Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation.

Review 1. Deep tissue two-photon microscopy.

2. Major signal increase in fluorescence microscopy through dark-state relaxation.

3. Simultaneous visualization of spatial and chromatic aberrations by two-dimensional Fourier transform spectral interferometry.

4. Quartic-phase-limited grism-based ultrashort pulse shaper.

5. Simultaneous multifocal, multiphoton, photon counting microscopy.

6. Effect of pulse shape on the efficiency of multiphoton processes: implications for biological microscopy.

7. Characterization of a High Efficiency, Ultrashort Pulse Shaper Incorporating a Reflective 4096-Element Spatial Light Modulator.

8. Dispersion pre-compensation of 15 femtosecond optical pulses for high-numerical-aperture objectives.

9. Two-photon laser scanning fluorescence microscopy.

10. Absolute two-photon absorption spectra and two-photon brightness of orange and red fluorescent proteins.

1. Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy.

2. A pragmatic guide to multiphoton microscope design.

3. Optimizing pulse compressibility in completely all-fibered Ytterbium chirped pulse amplifiers for in vivo two photon laser scanning microscopy.

4. Fiber-based tunable repetition rate source for deep tissue two-photon fluorescence microscopy.

5. Two-dimensional random access multiphoton spatial frequency modulated imaging.

Review 6. Two-photon absorption properties of fluorescent proteins.

7. Dependence of Two-Photon eGFP Bleaching on Femtosecond Pulse Spectral Amplitude and Phase.

8. Advances in multiphoton microscopy technology.

9. Remote focusing for programmable multi-layer differential multiphoton microscopy.

10. Multiphoton photochemistry of red fluorescent proteins in solution and live cells.