Literature DB >> 21471395

Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy.

Jonathan D Driscoll1, Andy Y Shih, Satish Iyengar, Jeffrey J Field, G Allen White, Jeffrey A Squier, Gert Cauwenberghs, David Kleinfeld.   

Abstract

We present a high-speed photon counter for use with two-photon microscopy. Counting pulses of photocurrent, as opposed to analog integration, maximizes the signal-to-noise ratio so long as the uncertainty in the count does not exceed the gain-noise of the photodetector. Our system extends this improvement through an estimate of the count that corrects for the censored period after detection of an emission event. The same system can be rapidly reconfigured in software for fluorescence lifetime imaging, which we illustrate by distinguishing between two spectrally similar fluorophores in an in vivo model of microstroke.

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Year:  2011        PMID： 21471395      PMCID： PMC3118755          DOI： 10.1152/jn.00649.2010

Source DB:  PubMed          Journal:  J Neurophysiol        ISSN： 0022-3077            Impact factor:   2.714


  39 in total

1.  Fast scanning and efficient photodetection in a simple two-photon microscope.

Authors:  Y P Tan; I Llano; A Hopt; F Würriehausen; E Neher
Journal:  J Neurosci Methods       Date:  1999-10-15       Impact factor: 2.390

Review 2.  Fluorescence lifetime measurements and biological imaging.

Authors:  Mikhail Y Berezin; Samuel Achilefu
Journal:  Chem Rev       Date:  2010-05-12       Impact factor: 60.622

3.  Topological basis for the robust distribution of blood to rodent neocortex.

Authors:  Pablo Blinder; Andy Y Shih; Christopher Rafie; David Kleinfeld
Journal:  Proc Natl Acad Sci U S A       Date:  2010-06-28       Impact factor: 11.205

Review 4.  Deep tissue two-photon microscopy.

Authors:  Fritjof Helmchen; Winfried Denk
Journal:  Nat Methods       Date:  2005-12       Impact factor: 28.547

5.  Penetrating arterioles are a bottleneck in the perfusion of neocortex.

Authors:  Nozomi Nishimura; Chris B Schaffer; Beth Friedman; Patrick D Lyden; David Kleinfeld
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-26       Impact factor: 11.205

6.  In vivo dendritic calcium dynamics in neocortical pyramidal neurons.

Authors:  K Svoboda; W Denk; D Kleinfeld; D W Tank
Journal:  Nature       Date:  1997-01-09       Impact factor: 49.962

7.  Two-photon laser scanning fluorescence microscopy.

Authors:  W Denk; J H Strickler; W W Webb
Journal:  Science       Date:  1990-04-06       Impact factor: 47.728

8.  Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy.

Authors:  Richard K P Benninger; William J Ashby; Elisabeth A Ring; David W Piston
Journal:  Opt Lett       Date:  2008-12-15       Impact factor: 3.776

9.  Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window.

Authors:  Anthony Holtmaat; Tobias Bonhoeffer; David K Chow; Jyoti Chuckowree; Vincenzo De Paola; Sonja B Hofer; Mark Hübener; Tara Keck; Graham Knott; Wei-Chung A Lee; Ricardo Mostany; Tom D Mrsic-Flogel; Elly Nedivi; Carlos Portera-Cailliau; Karel Svoboda; Joshua T Trachtenberg; Linda Wilbrecht
Journal:  Nat Protoc       Date:  2009-07-16       Impact factor: 13.491

10.  Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo.

Authors:  Christoph J Engelbrecht; Richard S Johnston; Eric J Seibel; Fritjof Helmchen
Journal:  Opt Express       Date:  2008-04-14       Impact factor: 3.894
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  17 in total

Review 1.  Two-photon microscopy as a tool to study blood flow and neurovascular coupling in the rodent brain.

Authors:  Andy Y Shih; Jonathan D Driscoll; Patrick J Drew; Nozomi Nishimura; Chris B Schaffer; David Kleinfeld
Journal:  J Cereb Blood Flow Metab       Date:  2012-02-01       Impact factor: 6.200

2.  Fluctuating and sensory-induced vasodynamics in rodent cortex extend arteriole capacity.

Authors:  Patrick J Drew; Andy Y Shih; David Kleinfeld
Journal:  Proc Natl Acad Sci U S A       Date:  2011-05-02       Impact factor: 11.205

3.  Central Amygdala Somatostatin Neurons Gate Passive and Active Defensive Behaviors.

Authors:  Kai Yu; Pedro Garcia da Silva; Dinu F Albeanu; Bo Li
Journal:  J Neurosci       Date:  2016-06-15       Impact factor: 6.167

4.  A pragmatic guide to multiphoton microscope design.

Authors:  Michael D Young; Jeffrey J Field; Kraig E Sheetz; Randy A Bartels; Jeff Squier
Journal:  Adv Opt Photonics       Date:  2015-06-30       Impact factor: 20.107

5.  Direct wavefront sensing enables functional imaging of infragranular axons and spines.

Authors:  Rui Liu; Zengyi Li; Jonathan S Marvin; David Kleinfeld
Journal:  Nat Methods       Date:  2019-06-17       Impact factor: 28.547

Review 6.  High-resolution in vivo optical imaging of stroke injury and repair.

Authors:  Sava Sakadžić; Jonghwan Lee; David A Boas; Cenk Ayata
Journal:  Brain Res       Date:  2015-05-08       Impact factor: 3.252

7.  Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging.

Authors:  Claudio Vinegoni; Paolo Fumene Feruglio; Christian Brand; Sungon Lee; Antoinette E Nibbs; Shawn Stapleton; Sunil Shah; Ignacy Gryczynski; Thomas Reiner; Ralph Mazitschek; Ralph Weissleder
Journal:  Nat Protoc       Date:  2017-06-29       Impact factor: 13.491

8.  Advances in multiphoton microscopy technology.

Authors:  Erich E Hoover; Jeff A Squier
Journal:  Nat Photonics       Date:  2013-02-01       Impact factor: 38.771

9.  Steady state anisotropy two-photon microscopy resolves multiple, spectrally similar fluorophores, enabling in vivo multilabel imaging.

Authors:  J Matthew Dubach; Claudio Vinegoni; Ralph Weissleder
Journal:  Opt Lett       Date:  2014-08-01       Impact factor: 3.776

10.  Ultrafast photon counting applied to resonant scanning STED microscopy.

Authors:  Xundong Wu; Ligia Toro; Enrico Stefani; Yong Wu
Journal:  J Microsc       Date:  2014-09-16       Impact factor: 1.758
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