CONTEXT: Whole-slide images (WSI) are a tool for remote interpretation, archiving, and teaching. Ovarian frozen sections (FS) are common and hence determination of the operating characteristics of the interpretation of these specimens using WSI is important. OBJECTIVES: To test the reproducibility and accuracy of ovarian FS interpretation using WSI, as compared with routine analog interpretation, to understand the technology limits and unique interpretive pitfalls. DESIGN: A sequential series of ovarian FS slides, representative of routine practice, were converted to WSI. Whole-slide images were examined by 2 pathologists, masked to all prior results. Correlation characteristics among the WSI, the original, and the final interpretations were analyzed. RESULTS: A total of 52 cases, consisting of 71 FS slides, were included; 34 cases (65%) were benign, and 18 cases (35%) were malignant, borderline, and of uncertain potential (9 [17%], 7 [13%], and 2 [4%] of 52 cases, respectively). The correlation between WSI and FS interpretations was 96% (50 of 52) for each pathologist for benign versus malignant, borderline, and uncertain entities. Each pathologist undercalled 2 borderline malignant cases (4%) as benign cysts on WSI. There were no overcalls of benign cases. Specific issues within the benign and malignant groups involved endometriosis versus hemorrhagic corpora lutea, and granulosa cell tumor versus carcinoma, respectively. CONCLUSIONS: The correlation between original FS and WSI interpretations was very high. The few discordant cases represent recognized differential diagnostic issues. Ability to examine gross pathology and real-time consultation with surgeons might be expected to improve performance. Ovarian FS diagnosis by WSI is accurate and reproducible, and thus, remote interpretation, teaching, and digital archiving of ovarian FS specimens by this method can be reliable.
CONTEXT: Whole-slide images (WSI) are a tool for remote interpretation, archiving, and teaching. Ovarian frozen sections (FS) are common and hence determination of the operating characteristics of the interpretation of these specimens using WSI is important. OBJECTIVES: To test the reproducibility and accuracy of ovarian FS interpretation using WSI, as compared with routine analog interpretation, to understand the technology limits and unique interpretive pitfalls. DESIGN: A sequential series of ovarian FS slides, representative of routine practice, were converted to WSI. Whole-slide images were examined by 2 pathologists, masked to all prior results. Correlation characteristics among the WSI, the original, and the final interpretations were analyzed. RESULTS: A total of 52 cases, consisting of 71 FS slides, were included; 34 cases (65%) were benign, and 18 cases (35%) were malignant, borderline, and of uncertain potential (9 [17%], 7 [13%], and 2 [4%] of 52 cases, respectively). The correlation between WSI and FS interpretations was 96% (50 of 52) for each pathologist for benign versus malignant, borderline, and uncertain entities. Each pathologist undercalled 2 borderline malignant cases (4%) as benign cysts on WSI. There were no overcalls of benign cases. Specific issues within the benign and malignant groups involved endometriosis versus hemorrhagic corpora lutea, and granulosa cell tumor versus carcinoma, respectively. CONCLUSIONS: The correlation between original FS and WSI interpretations was very high. The few discordant cases represent recognized differential diagnostic issues. Ability to examine gross pathology and real-time consultation with surgeons might be expected to improve performance. Ovarian FS diagnosis by WSI is accurate and reproducible, and thus, remote interpretation, teaching, and digital archiving of ovarian FS specimens by this method can be reliable.
Authors: Anna Luíza Damaceno Araújo; Gleyson Kleber Amaral-Silva; Felipe Paiva Fonseca; Natália Rangel Palmier; Marcio Ajudarte Lopes; Paul M Speight; Oslei Paes de Almeida; Pablo Agustin Vargas; Alan Roger Santos-Silva Journal: Virchows Arch Date: 2018-06-01 Impact factor: 4.064
Authors: Anna Luíza Damaceno Araújo; Lady Paola Aristizábal Arboleda; Natalia Rangel Palmier; Jéssica Montenegro Fonsêca; Mariana de Pauli Paglioni; Wagner Gomes-Silva; Ana Carolina Prado Ribeiro; Thaís Bianca Brandão; Luciana Estevam Simonato; Paul M Speight; Felipe Paiva Fonseca; Marcio Ajudarte Lopes; Oslei Paes de Almeida; Pablo Agustin Vargas; Cristhian Camilo Madrid Troconis; Alan Roger Santos-Silva Journal: Virchows Arch Date: 2019-01-26 Impact factor: 4.064
Authors: David C Wilbur; Kalil Madi; Robert B Colvin; Lyn M Duncan; William C Faquin; Judith A Ferry; Matthew P Frosch; Stuart L Houser; Richard L Kradin; Gregory Y Lauwers; David N Louis; Eugene J Mark; Mari Mino-Kenudson; Joseph Misdraji; Gunnlauger P Nielsen; Martha B Pitman; Andrew E Rosenberg; R Neal Smith; Aliyah R Sohani; James R Stone; Rosemary H Tambouret; Chin-Lee Wu; Robert H Young; Artur Zembowicz; Wolfgang Klietmann Journal: Arch Pathol Lab Med Date: 2009-12 Impact factor: 5.534
Authors: Liron Pantanowitz; John H Sinard; Walter H Henricks; Lisa A Fatheree; Alexis B Carter; Lydia Contis; Bruce A Beckwith; Andrew J Evans; Avtar Lal; Anil V Parwani Journal: Arch Pathol Lab Med Date: 2013-05-01 Impact factor: 5.534
Authors: M Babawale; A Gunavardhan; J Walker; T Corfield; P Huey; A Savage; A Bansal; M Atkinson; H Abdelsalam; E Raweily; A Christian; I Evangelou; D Thomas; J Shannon; E Youd; P Brumwell; J Harrison; I Thompson; M Rashid; G Leopold; A Finall; S Roberts; D Housa; P Nedeva; A Davies; D Fletcher; Muhammad Aslam Journal: J Pathol Inform Date: 2021-01-23