Literature DB >> 2057368

Eliminating primers from completed polymerase chain reactions with exonuclease VII.

H H Li1, X F Cui, N Arnheim.   

Abstract

Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR product are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols.

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Year:  1991        PMID： 2057368      PMCID： PMC328282          DOI： 10.1093/nar/19.11.3139

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

2.  Single-sperm typing: determination of genetic distance between the G gamma-globin and parathyroid hormone loci by using the polymerase chain reaction and allele-specific oligomers.

Authors:  X F Cui; H H Li; T M Goradia; K Lange; H H Kazazian; D Galas; N Arnheim
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

3.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

4.  Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.

Authors:  K B Mullis; F A Faloona
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  Exonuclease VII of Escherichia coli. Mechanism of action.

Authors:  J W Chase; C C Richardson
Journal:  J Biol Chem       Date:  1974-07-25       Impact factor: 5.157

6.  Exonuclease VII of Escherichia coli. Purification and properties.

Authors:  J W Chase; C C Richardson
Journal:  J Biol Chem       Date:  1974-07-25       Impact factor: 5.157

7.  Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.

Authors:  H Li; X Cui; N Arnheim
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205
  7 in total
  2 in total

1.  Enzymes used in molecular biology: a useful guide.

Authors:  Laure Rittié; Bernard Perbal
Journal:  J Cell Commun Signal       Date:  2008-09-03       Impact factor: 5.782

2.  Dilute-'N'-Go dideoxy sequencing of all DNA strands generated in multiplex LATE-PCR assays.

Authors:  Yanwei Jia; Adam Osborne; John E Rice; Lawrence J Wangh
Journal:  Nucleic Acids Res       Date:  2010-02-26       Impact factor: 16.971
  2 in total

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