| Literature DB >> 20570578 |
Kinta M Serve1, Jennifer L Darnell, Jody K Takemoto, Neal M Davies, Margaret E Black.
Abstract
The efficacy of the chemotherapeutic drug 5'-fluorouracil is reduced by catabolism to 2'-fluoro-beta-alanine (FBAL), a three-step reaction in which dihydropyrimidine dehydrogenase (DPD) catalyzes the rate-limiting step. To study in vitro DPD activity, we developed and validated an isocratic, reverse-phase HPLC method to detect and quantify FBAL without using multiple columns or radiolabeled substrates. Pre-column derivatization of FBAL was performed using o-phthalaldehyde in the presence of two sulfur donors, ethanthiol or beta-mercaptoethanol, and the resulting products assayed. Calibration curves were linear over a range of 10-200 microg/ml and the method was successfully applied to the examination of DPD activity in cultured cells. Copyright 2010 Elsevier B.V. All rights reserved.Entities:
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Year: 2010 PMID： 20570578 PMCID： PMC2902665 DOI： 10.1016/j.jchromb.2010.05.010
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205