| Literature DB >> 20552434 |
Youn-Kyung Kim1, Loredana Quadro.
Abstract
The ability to measure endogenous metabolites of retinoids (vitamin A and its derivatives) in biological samples is key to understanding the crucial physiological actions of vitamin A. Over the years, many assays and methods have been developed to analyze different retinoids in biological samples. Liquid chromatography is the best analytical technique for routine analysis of these compounds. However, due to their different chemical properties, different retinoid metabolites cannot be accurately separated and quantified in a single chromatographic run. Here, we will describe a reverse-phase HPLC isocratic method that enables extraction, separation, identification, and quantification of all-trans-retinol and different molecular species of retinyl ester with high accuracy, sensitivity, and reliability.Entities:
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Year: 2010 PMID： 20552434 PMCID： PMC3716261 DOI： 10.1007/978-1-60327-325-1_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745