Literature DB >> 20543052

Determining UV inactivation of Toxoplasma gondii oocysts by using cell culture and a mouse bioassay.

Michael W Ware1, Swinburne A J Augustine, David O Erisman, Mary Jean See, Larry Wymer, Samuel L Hayes, J P Dubey, Eric N Villegas.   

Abstract

The effect of UV exposure on Toxoplasma gondii oocysts has not been completely defined for use in water disinfection. This study evaluated UV-irradiated oocysts by three assays: a SCID mouse bioassay, an in vitro T. gondii oocyst plaque (TOP) assay, and a quantitative reverse transcriptase real-time PCR (RT-qPCR) assay. The results from the animal bioassay show that 1- and 3-log(10) inactivation is achieved with 4 mJ/cm(2) UV and 10 mJ/cm(2) low-pressure UV, respectively. TOP assay results, but not RT-qPCR results, correlate well with bioassay results. In conclusion, a 3-log(10) inactivation of T. gondii oocysts is achieved by 10-mJ/cm(2) low-pressure UV, and the in vitro TOP assay is a promising alternative to the mouse bioassay.

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Year:  2010        PMID： 20543052      PMCID： PMC2916465          DOI： 10.1128/AEM.00153-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  54 in total

1.  Severe acquired toxoplasmosis in immunocompetent adult patients in French Guiana.

Authors:  B Carme; F Bissuel; D Ajzenberg; R Bouyne; C Aznar; M Demar; S Bichat; D Louvel; A M Bourbigot; C Peneau; P Neron; M L Dardé
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

2.  Toxoplasma gondii infection in the United States: seroprevalence and risk factors.

Authors:  J L Jones; D Kruszon-Moran; M Wilson; G McQuillan; T Navin; J B McAuley
Journal:  Am J Epidemiol       Date:  2001-08-15       Impact factor: 4.897

3.  Low-pressure UV inactivation and DNA repair potential of Cryptosporidium parvum oocysts.

Authors:  G A Shin; K G Linden; M J Arrowood; M D Sobsey
Journal:  Appl Environ Microbiol       Date:  2001-07       Impact factor: 4.792

4.  Estimating viability of Cryptosporidium parvum oocysts using reverse transcriptase-polymerase chain reaction (RT-PCR) directed at mRNA encoding amyloglucosidase.

Authors:  M C Jenkins; J Trout; M S Abrahamsen; C A Lancto; J Higgins; R Fayer
Journal:  J Microbiol Methods       Date:  2000-12-15       Impact factor: 2.363

5.  Comparison of in vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum.

Authors:  Paul A Rochelle; Marilyn M Marshall; Jan R Mead; Anne M Johnson; Dick G Korich; Jeffrey S Rosen; Ricardo De Leon
Journal:  Appl Environ Microbiol       Date:  2002-08       Impact factor: 4.792

6.  Fatal outbreak of human toxoplasmosis along the Maroni River: epidemiological, clinical, and parasitological aspects.

Authors:  Magalie Demar; Daniel Ajzenberg; Danielle Maubon; Félix Djossou; Dayanand Panchoe; Widya Punwasi; Nasser Valery; Christian Peneau; Jean-Louis Daigre; Christine Aznar; Benoit Cottrelle; Laurence Terzan; Marie-Laure Dardé; Bernard Carme
Journal:  Clin Infect Dis       Date:  2007-08-27       Impact factor: 9.079

7.  Coastal freshwater runoff is a risk factor for Toxoplasma gondii infection of southern sea otters (Enhydra lutris nereis).

Authors:  M A Miller; I A Gardner; C Kreuder; D M Paradies; K R Worcester; D A Jessup; E Dodd; M D Harris; J A Ames; A E Packham; P A Conrad
Journal:  Int J Parasitol       Date:  2002-07       Impact factor: 3.981

8.  Low pressure ultraviolet studies for inactivation of Giardia muris cysts.

Authors:  S L Hayes; E W Rice; M W Ware; F W Schaefer
Journal:  J Appl Microbiol       Date:  2003       Impact factor: 3.772

9.  Development of a PCR-enzyme immunoassay oligoprobe detection method for Toxoplasma gondii oocysts, incorporating PCR controls.

Authors:  Kellogg J Schwab; James J McDevitt
Journal:  Appl Environ Microbiol       Date:  2003-10       Impact factor: 4.792

10.  Highly endemic, waterborne toxoplasmosis in north Rio de Janeiro state, Brazil.

Authors:  Lílian Maria Garcia Bahia-Oliveira; Jeffrey L Jones; Juliana Azevedo-Silva; Cristiane C F Alves; Fernando Oréfice; David G Addiss
Journal:  Emerg Infect Dis       Date:  2003-01       Impact factor: 6.883
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  14 in total

Review 1.  A review on inactivation methods of Toxoplasma gondii in foods.

Authors:  Adel Mirza Alizadeh; Sahar Jazaeri; Bahar Shemshadi; Fataneh Hashempour-Baltork; Zahra Sarlak; Zahra Pilevar; Hedayat Hosseini
Journal:  Pathog Glob Health       Date:  2018-10-22       Impact factor: 2.894

Review 2.  Inactivation of exogenous endoparasite stages by chemical disinfectants: current state and perspectives.

Authors:  Arwid Daugschies; Berit Bangoura; Matthias Lendner
Journal:  Parasitol Res       Date:  2013-02-08       Impact factor: 2.289

3.  Oral inoculation of ultraviolet-irradiated Eimeria species oocysts protects chickens against coccidiosis.

Authors:  Saeed A El-Ashram; Shawky M Aboelhadid; Sahar M Gadelhaq; Walid M Arafa; Abdel-Razik H Abdel-Razik; Salama Abohamra; Khaled T Abdelaziz
Journal:  Parasitol Res       Date:  2019-10-12       Impact factor: 2.289

4.  The development and implementation of a method using blue mussels (Mytilus spp.) as biosentinels of Cryptosporidium spp. and Toxoplasma gondii contamination in marine aquatic environments.

Authors:  Sarah E Staggs; Scott P Keely; Michael W Ware; Nancy Schable; Mary Jean See; Dominic Gregorio; Xuan Zou; Chunlei Su; J P Dubey; Eric N Villegas
Journal:  Parasitol Res       Date:  2015-09-11       Impact factor: 2.289

5.  Mechanics of the Toxoplasma gondii oocyst wall.

Authors:  Aurélien Dumètre; Jitender P Dubey; David J P Ferguson; Pierre Bongrand; Nadine Azas; Pierre-Henri Puech
Journal:  Proc Natl Acad Sci U S A       Date:  2013-06-24       Impact factor: 11.205

6.  Toxoplasma gondii Oocyst Infectivity Assessed Using a Sporocyst-Based Cell Culture Assay Combined with Quantitative PCR for Environmental Applications.

Authors:  Angélique Rousseau; Sandie Escotte-Binet; Stéphanie La Carbona; Aurélien Dumètre; Sophie Chagneau; Loïc Favennec; Sophie Kubina; Jitender P Dubey; Didier Majou; Aurélie Bigot-Clivot; Isabelle Villena; Dominique Aubert
Journal:  Appl Environ Microbiol       Date:  2019-10-01       Impact factor: 4.792

7.  Viability of Pseudocapillaria tomentosa Eggs Exposed to Heat, Ultraviolet Light, Chlorine, Iodine, and Desiccation.

Authors:  Michael L Kent; Virginia Watral; Eric N Villegas; Christopher A Gaulke
Journal:  Zebrafish       Date:  2019-06-19       Impact factor: 1.985

8.  Evaluation of propidium monoazide-based qPCR to detect viable oocysts of Toxoplasma gondii.

Authors:  Angélique Rousseau; Isabelle Villena; Aurélien Dumètre; Sandie Escotte-Binet; Loïc Favennec; Jitender P Dubey; Dominique Aubert; Stéphanie La Carbona
Journal:  Parasitol Res       Date:  2019-02-07       Impact factor: 2.289

9.  First report of Toxoplasma gondii sporulated oocysts and Giardia duodenalis in commercial green-lipped mussels (Perna canaliculus) in New Zealand.

Authors:  Alicia Coupe; Laryssa Howe; Elizabeth Burrows; Abigail Sine; Anthony Pita; Niluka Velathanthiri; Emilie Vallée; David Hayman; Karen Shapiro; Wendi D Roe
Journal:  Parasitol Res       Date:  2018-03-17       Impact factor: 2.289

10.  Drinking water source and human Toxoplasma gondii infection in the United States: a cross-sectional analysis of NHANES data.

Authors:  Whitney S Krueger; Elizabeth D Hilborn; Reagan R Converse; Timothy J Wade
Journal:  BMC Public Health       Date:  2014-07-10       Impact factor: 3.295
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