BACKGROUND & OBJECTIVES: Dengue is currently one of the most important arthropod-borne diseases and may be caused by four different dengue virus serotypes (DENV-1 to DENV-4), transmitted mainly by Aedes aegypti (Diptera: Culicidae) mosquitoes. With the lack of a dengue vaccine, vector control strategies constitute a crucial mode to prevent or reduce disease transmission. In this context, DENV detection in natural Ae. aegypti populations may serve as a potential additional tool for early prediction systems of dengue outbreaks, leading to an intensification of vector control measures, aimed at reducing disease transmission. In Brazil, this type of surveillance has been performed sporadically by a few groups and has not been incorporated as a routine activity in control programs. This study aimed at detecting DENV in natural Ae. aegypti from Recife, Pernambuco, to check the circulating serotypes and the occurrence of transovarial transmission in local mosquito populations. METHODS: From January 2005 to June 2006, mosquitoes (adults and eggs) were collected in houses where people with clinical suspicion of dengue infection lived at. RNA was extracted from pooled mosquitoes and RT-PCR was performed in these samples for detection of the four DENV serotypes. RESULTS & CONCLUSION: Out of 83 pools of adult mosquitoes collected in the field, nine were positive for DENV: five for DENV-1, two for DENV-2 and two for DENV-3. From 139 pools of adult mosquitoes reared from collected eggs, there were 17 positive pools: three for DENV-1, 10 for DENV-2, and four for DENV-3. These results are discussed in the paper in regard to the local dengue epidemiological data. The conclusions clearly point to the informative power and sensitivity of DENV entomological surveillance and to the importance of including mosquito immature forms in this strategy.
BACKGROUND & OBJECTIVES: Dengue is currently one of the most important arthropod-borne diseases and may be caused by four different dengue virus serotypes (DENV-1 to DENV-4), transmitted mainly by Aedes aegypti (Diptera: Culicidae) mosquitoes. With the lack of a dengue vaccine, vector control strategies constitute a crucial mode to prevent or reduce disease transmission. In this context, DENV detection in natural Ae. aegypti populations may serve as a potential additional tool for early prediction systems of dengue outbreaks, leading to an intensification of vector control measures, aimed at reducing disease transmission. In Brazil, this type of surveillance has been performed sporadically by a few groups and has not been incorporated as a routine activity in control programs. This study aimed at detecting DENV in natural Ae. aegypti from Recife, Pernambuco, to check the circulating serotypes and the occurrence of transovarial transmission in local mosquito populations. METHODS: From January 2005 to June 2006, mosquitoes (adults and eggs) were collected in houses where people with clinical suspicion of dengue infection lived at. RNA was extracted from pooled mosquitoes and RT-PCR was performed in these samples for detection of the four DENV serotypes. RESULTS & CONCLUSION: Out of 83 pools of adult mosquitoes collected in the field, nine were positive for DENV: five for DENV-1, two for DENV-2 and two for DENV-3. From 139 pools of adult mosquitoes reared from collected eggs, there were 17 positive pools: three for DENV-1, 10 for DENV-2, and four for DENV-3. These results are discussed in the paper in regard to the local dengue epidemiological data. The conclusions clearly point to the informative power and sensitivity of DENV entomological surveillance and to the importance of including mosquito immature forms in this strategy.
Authors: Lars Eisen; Julián E García-Rejón; Salvador Gómez-Carro; María del Rosario Nájera Vázquez; Thomas J Keefe; Barry J Beaty; María Alba Loroño-Pino Journal: J Med Entomol Date: 2014-07 Impact factor: 2.278
Authors: Julián E García-Rejón; María Alba Loroño-Pino; José Arturo Farfán-Ale; Luis F Flores-Flores; Mildred P López-Uribe; Maria Del Rosario Najera-Vazquez; Guadalupe Nuñez-Ayala; Barry J Beaty; Lars Eisen Journal: Am J Trop Med Hyg Date: 2011-03 Impact factor: 2.345
Authors: María Alba Loroño-Pino; Julián E García-Rejón; Carlos Machain-Williams; Salvador Gomez-Carro; Guadalupe Nuñez-Ayala; Maria del Rosario Nájera-Vázquez; Arturo Losoya; Lyla Aguilar; Karla Saavedra-Rodriguez; Saul Lozano-Fuentes; Meaghan K Beaty; William C Black; Thomas J Keefe; Lars Eisen; Barry J Beaty Journal: Am J Trop Med Hyg Date: 2013-06-03 Impact factor: 2.345
Authors: Victor Emanuel Pessoa Martins; Carlos Henrique Alencar; Michel Toth Kamimura; Michel Tott Kamimura; Fernanda Montenegro de Carvalho Araújo; Salvatore Giovanni De Simone; Rosa Fireman Dutra; Maria Izabel Florindo Guedes Journal: PLoS One Date: 2012-07-25 Impact factor: 3.240
Authors: Rosalía Pérez-Castro; Jaime E Castellanos; Víctor A Olano; María Inés Matiz; Juan F Jaramillo; Sandra L Vargas; Diana M Sarmiento; Thor Axel Stenström; Hans J Overgaard Journal: Mem Inst Oswaldo Cruz Date: 2016-04 Impact factor: 2.743
Authors: Víctor Hugo Peña-García; Omar Triana-Chávez; Ana María Mejía-Jaramillo; Francisco J Díaz; Andrés Gómez-Palacio; Sair Arboleda-Sánchez Journal: Int J Environ Res Public Health Date: 2016-07-21 Impact factor: 3.390