Literature DB >> 20530883

Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus.

Chun Zhang1, Xiaolan Yang, Juan Feng, Yonghua Yuan, Xiang Li, Youquan Bu, Yanling Xie, Huidong Yuan, Fei Liao.   

Abstract

After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).

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Year:  2010        PMID： 20530883     DOI： 10.1271/bbb.100080

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  7 in total

1.  Uricases as therapeutic agents to treat refractory gout: Current states and future directions.

Authors:  Xiaolan Yang; Yonghua Yuan; Chang-Guo Zhan; Fei Liao
Journal:  Drug Dev Res       Date:  2011-12-29       Impact factor: 4.360

2.  Optimized UV-Spectrophotometric Assay to Screen Bacterial Uricase Activity Using Whole Cell Suspension.

Authors:  Benoit Pugin; Serafina Plüss; Denisa Mujezinovic; Rikke C Nielsen; Christophe Lacroix
Journal:  Front Microbiol       Date:  2022-04-13       Impact factor: 6.064

3.  Catalytic Mechanisms for Cofactor-Free Oxidase-Catalyzed Reactions: Reaction Pathways of Uricase-Catalyzed Oxidation and Hydration of Uric Acid.

Authors:  Donghui Wei; Xiaoqin Huang; Yan Qiao; Jingjing Rao; Lu Wang; Fei Liao; Chang-Guo Zhan
Journal:  ACS Catal       Date:  2017-06-15       Impact factor: 13.084

4.  Extracorporeal delivery of a therapeutic enzyme.

Authors:  Chun Zhang; Jun Pu; Xiaolan Yang; Tao Feng; Fang Liu; Deqiang Wang; Xiaolei Hu; Ang Gao; Hongbo Liu; Chang-Guo Zhan; Fei Liao
Journal:  Sci Rep       Date:  2016-08-01       Impact factor: 4.379

5.  Improved biological properties and hypouricemic effects of uricase from Candida utilis loaded in novel alkaline enzymosomes.

Authors:  Qun-You Tan; Jing-Qing Zhang; Na Wang; Hong Yang; Xiaoling Li; Hua-Rong Xiong; Jian-Yong Wu; Chun-Jing Zhao; Hong Wang; Hua-Feng Yin
Journal:  Int J Nanomedicine       Date:  2012-07-23

6.  Comparison of activity indexes for recognizing enzyme mutants of higher activity with uricase as model.

Authors:  Juan Feng; Hongbo Liu; Xiaolan Yang; Ang Gao; Juan Liao; Liping Feng; Jun Pu; Yanling Xie; Gaobo Long; Yuanli Li; Fei Liao
Journal:  Chem Cent J       Date:  2013-04-17       Impact factor: 4.215

7.  Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives.

Authors:  Ang Gao; Xiaolan Yang; Chun Zhang; Gaobo Long; Jun Pu; Yonghua Yuan; Hongbo Liu; Yuanli Li; Fei Liao
Journal:  Chem Cent J       Date:  2012-11-23       Impact factor: 4.215
  7 in total

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