Nosocomial methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in Taiwan: mortality analyses and the impact of vancomycin, MIC = 2 mg/L, by the broth microdilution method.

BACKGROUND: Previous studies regarding the prognosis of patients infected with MRSA isolates characterized by a high minimum inhibitory concentration (MIC) for vancomycin have generally used a commercial Etest. Little research has been conducted on determining the vancomycin susceptibility of MRSA using a reference microdilution. Additionally, there is discordance between the MIC result from an Etest and the value determined using the reference microdilution method.
METHODS: Using a reference microdilution method, we determined the MIC of vancomycin for isolates from 123 consecutive patients with nosocomial MRSA bacteremia. The clinical features and outcome for these patients were recorded and the MRSA isolates were genotyped.
RESULTS: Among the 123 non-duplicated isolates, 21.1% had a MIC = 2 mg/L, 76.4% had a MIC = 1 mg/L and 2.4% had MIC = 0.5 mg/L. Patients with MRSA bacteremia in the ICU or those who had been hospitalized for a long time were more likely to be infected with strains of high vancomycin MIC MRSA (MIC = 2 mg/L; p < 0.05). Cox regression analysis demonstrated that the high MIC group had a significantly higher 30-day mortality than the low MIC group (HR: 2.39; 95% CI: 1.20-4.79; p = 0.014). Multivariate analyses indicated that the presence of high MIC isolates, pneumonia, post-cardiothoracic surgery and a high Charlson comorbidity index were all independent predictors of a 30-day mortality. Genotyping of these high vancomycin MIC isolates demonstrated that SCCmec III, spa type037, was the predominant strain (> 80%). The rates of resistance to trimethoprim/sulfamethoxazole, gentamicin, levofloxacin, rifampin and tetracycline were also higher in the high MIC group than in the isolates belonging to low MIC group (p < 0.05).
CONCLUSIONS: In a high vancomycin MIC group in Taiwan, SCCmec III, spa type t037, was the predominant strain of MRSA identified. Patients with MRSA bacteremia in the ICU or who had prolonged hospitalization were more likely to be infected with S. aureus strains with high vancomycin MICs. The mortality rate was higher among patients infected with these strains compared to patients infected with low MIC strains.

Background

In 2004, Sakoulas et al. observed that a significant risk for vancomycin treatment failure in MRSA bacteremia is first indicated by increasing vancomycin MICs that are well within the susceptible range [1]. Most of the studies examining the impact of high vancomycin MIC in patients with MRSA bacteremia published after the Sakoulas et al. report have used the commercial E test and found similar results [2-4]. However, a recent study, using multivariate analysis, demonstrated that there was no difference in mortality between high MIC and low MIC patient groups [5]. There were discordant MIC results between different susceptibility methods [6-8]. Furthermore, there has been little research examining the use of reference microdilution as a method of vancomycin susceptibility determination [9].

Additionally, a previous genotype study in the U.S. showed that SCCmec II was the genotype that was most predictive of high vancomycin MIC isolates [10]. In Taiwan, the molecular epidemiology of isolates from patients with MRSA bacteremia is distinct [11]. We do not know if the high vancomycin MIC isolates from patients in Taiwan have a similar genotype as in those seen in patients in the U.S.

Therefore, we collected bacteria from consecutive patients with nosocomial MRSA bacteremia and, using the Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution, determined vancomycin MICs. We examined the association of infection with high MIC strains (MIC = 2 mg/L) and mortality and genotyped the isolates.

Methods

This study was approved by the local institutional ethics review board (expedited review). The Institutional Review Board waived the need for informed consent from participants because the study involved very minimal risk to the subjects, did not include intentional deception and did not involve sensitive populations or topics; this waiver does not adversely affect the rights and welfare of the subjects. Throughout the one-year study period (January 1 to December 31, 2006), we collected clinical data from 123 non-duplicated, consecutive nosocomial MRSA bacteremia patients from the National Taiwan University Hospital in Taipei, Taiwan. During the study period, vancomycin and teicoplanin were the recommended antibiotics for treating MRSA bacteremia at our institutions. All patients were evaluated using a structured recording form. The clinical course of infection and the infection focus were evaluated and recorded according to information supplied by primary care physicians and medical records.

Identification of the infection focus was based on clinical, bacteriological and radiological investigations, and was defined according to criteria established by the Centers for Disease Control and Prevention [12]. We assessed patient survival rates 30 days after the diagnosis of bacteremia by follow-up at outpatient clinics or by telephone for patients who did not come to the outpatient clinics.

The vancomycin MIC of the 123 MRSA isolates was determined by broth microdilution, as described by the CLSI in 2005 [13]. In vitro testing of these isolates was performed in a blinded fashion without knowledge of any clinical outcomes. Isolates with vancomycin MIC = 2 mg/L were designated the "high MIC" group; those with MIC = 1 mg/L or 0.5 mg/L were considered to be the "low MIC" group. The susceptibility of the S. aureus isolates to levofloxacin, erythromycin, tetracycline, trimethoprim/sulfamethoxazole, gentamicin, clindamycin and rifampin was determined according to standard microbiological methods [13]. The presence of the SCCmec elements (I-V) was determined by previously described methods [14,15]. The polymorphic X-region of the protein A gene (spa) was analyzed as previously described [16].

Percentages were used for all categorical variables. For univariate analysis, we compared the high MIC and low MIC groups with a χ2 test or Fisher's exact test. We used multivariate logistic regression analyses to determine associations between potential risk factors and the presence of isolates with a high or low vancomycin MIC. The cumulative survival time after the first MRSA positive blood culture was calculated using the Kaplan-Meier method. The difference in cumulative survival of patients infected with high or low vancomycin MIC S. aureus was determined using the log-rank test. The effect of high MIC isolates on patient outcome was evaluated using a multivariate Cox proportional hazards regression model and a logistic regression model adjusted for age, sex and underlying comorbidities. Data were analyzed using SPSS software for Windows (Release 15.0; SPSS, Chicago, IL).

Results

Among the 123 non-duplicated isolates, 21.1% had a MIC = 2 mg/L, 76.4% had a MIC = 1 mg/L and 2.4% had a MIC = 0.5 mg/L (Table 1). Univariate analysis indicated that patients with MRSA bacteremia who were in the ICU (p = 0.028) or who had been hospitalized for a long time, i.e., > 60 days, (p = 0.022) were more likely to be infected with MRSA strains with a high vancomycin MIC (MIC = 2 mg/L). Multivariate analysis indicated that this effect remained after adjusting for sex, age and the presence of underlying disease. There were no other significant demographic differences between the high MIC and low MIC groups (Table 1). The infection syndrome, treatment and outcome are shown in Table 2.

Table 1

Demographic characteristics of nosocomial bacteremia patients infected with high MIC or low MIC MRSA

Characteristic	Vancomycin MIC < 2 N = 97 Number of patients (%)	Vancomycin MIC = 2 N = 26 Number of patients (%)	p-valuea	p-valueb; OR (95% CI)
Elderly (>65 years old)	66 (68.0)	13 (50.0)	0.088
Age	70.32 ± 14.1	63.6 ± 18.0	0.250
Sex: male	68 (70.1)	14 (53.8)	0.118	0.04; 0.33 (0.11-0.95)
Diabetes	70 (72.2)	17 (65.4)	0.500
Cancer	30 (30.9)	11(43.2)	0.349
Congestive heart failure	14 (14.4)	4(15.4)	1.000
End stage renal disease	10 (10.3)	4 (15.4)	0.492
Liver cirrhosis	11 (11.3)	3 (11.5)	1.000
Cerebrovascular disease	24 (24.7)	3 (11.5)	0.188
Bedridden	24 (24.7)	7 (26.9)	0.804
Recent surgery	24 (24.7)	3 (11.5)	0.188	0.018; 0.15 (0.03-0.73)
Recent cardio-thoracic surgery	13 (13.4)	1 (3.8)	0.297
Hospitalization days before bacteremia	22.6 ± 10.1	23.7 ± 8.0	0.207
ICU admission before bacteremia	40 (41.2)	17 (65.4)	0.028	0.005; 4.83 (1.61-14.50)
Hospitalization > 2 months before bactermia	17 (17.5)	10 (38.5)	0.022	0.011; 4.56 (1.41-14.73)
Hospitalization > 1 month before bacteremia	41 (42.3)	15 (57.7)	0.187

a: p-value calculated using a χ2 test or Fisher's exact test

b: p-value as determined by logistic regression.

Table 2

Infection syndrome, treatment and outcome of nosocomial bacteremia in patients infected with high MIC MRSA or low MIC MRSA

Syndrome & treatment	Vancomycin MIC < 2 N = 97 Number of patients (%)	Vancomycin MIC = 2 N = 26 Number of patients (%)	p-valuea
Syndrome
Primary bacteremia	18 (18.6)	5 (19.2)	0.938
Pneumonia	32 (33.0)	9 (34.6)	0.876
Catheter related infection	40 (41.2)	12 (46.2)	0.652
Prosthesis	6(6.2)	0 (0)	0.341
Infective endocarditis	6 (6.2)	0 (0)	0.341
Treatment
Empirical glycopeptide use within 48 hours	71 (73.2)	19 (73.1)	1.000
Vancomycin trough level (mg/L)	14.9 ± 7.6 (n = 47)	13.5 ± 4.9 (n = 12)	0.176
Vancomycin trough level > 10 mg/L in first week	34 (72.3)	9 (75.0)	1.000
With a DNR orderb	26 (26.8)	11 (42.3)	0.151
Outcome
2 week death	14 (14.4)	8 (30.8)	0.081
30 days death	27 (27.8)	13 (50.0)	0.057

a: p-value calculated using a χ2 test or Fisher's exact test

b: Do not resuscitate

The relationship between MIC and Day 14 and Day 30 mortality is shown in Figure 1. Additionally, we used Cox regression and logistic regression to evaluate the relationship between vancomycin MIC and mortality. Univariate analyses indicated that the presence of high MIC isolates, malignancy, a high Charlson comorbidity index, bedridden status and admission to the ICU were predictors of mortality after 30 days (p < 0.05) (Table 3). Variables with a p-value < 0.20 in the univariate analyses were included in the subsequent multivariate Cox proportional hazards regression model. Multivariate analyses in combination with Cox regression indicated that the presence of high MIC isolates, pneumonia, history of cardiothoracic surgery and a high Charlson comorbidity index were independent predictors of mortality after 30 days (p < 0.05). Patients infected with high MIC isolates had a greater 30-day mortality rate than patients infected with low MIC isolates, as indicated by univariate analysis (hazard ratio (HR) = 2.20; 95% confidence interval (CI): 1.13-4.27; p = 0.021) and multivariate Cox regression analysis (HR = 2.39; 95% CI: 1.20-4.79; p = 0.014). By using stepwise logistic regression, the presence of high MIC isolates, cancer, a high Charlson comorbidity index and a history of cardiothoracic surgery were shown to be independent predictors of mortality after 30 days (p < 0.05) (Table 4).

Figure 1

Mortality in different vancomycin MIC group. The relationship between vancomycin MIC and 14- and 30-days mortality

Table 3

Univariate and multivariate analysis of risk factors associated with mortality of patients with nosocomial MRSA bacteremia using Cox regression

Characteristic	Univariate HR	95% CI	p-value	Multivariate HR	95% CI	p-value
High vancomycin MIC	2.20	1.13-4.27	0.021	2.39	1.20-4.79	0.014
Malignancy	2.73	1.45-5.11	0.002	2.09	0.99-4.43	0.053
Pneumonia	1.74	0.92-3.27	0.088	2.27	1.17-4.40	0.016
Cardiothoracic surgery	1.86	0.82-4.20	0.139	4.38	1.74-11.04	0.002
Charlson score	1.27	1.10-1.46	0.001	1.24	1.07-1.45	0.005
Endocarditis	2.24	2.24-6.31	0.126
Bedridden	2.63	1.03-6.71	0.044
Cerebrovascular disease	2.06	0.81-5.27	0.131
ICU admission	2.05	1.07-3.90	0.029

Note: Variables with a p < 0.2 in the univariate analyses were included in the subsequent multivariate Cox proportional hazards regression model.

Table 4

Univariate and multivariate analyses of risk factors associated with mortality in patients with nosocomial MRSA bacteremia using logistic regression

Characteristic	Univariate OR	95% CI	p-value	Multivariate ORa	95% CI	p-value
High vancomycin MIC	2.59	1.07-6.30	0.035	3.76	1.34-10.54	0.012
Malignancy	3.48	1.57-7.74	0.002	3.06	1.15-8.17	0.025
Pneumonia	1.82	0.83-3.99	0.137
Cardiothoracic surgery	2.30	0.75-7.09	0.146	5.66	1.52-21.12	0.010
Charlson score	1.27	1.06-1.52	0.009	1.25	1.01-1.55	0.045
Endocarditis	4.50	0.79-25.69	0.09
Bedridden	3.19	1.12-9.08	0.03
Cerebrovascular disease	2.52	0.88-7.26	.086
ICU admission	2.27	1.05-4.91	.037

a: Covariates having a p < 0.2 were included in the stepwise regression, and covariates with a higher p-value were discarded.

The 30-day cumulative survival was 72.2% for patients infected with low MIC isolates and was 50% for patients infected with high MIC isolates (Figure 2). A log-rank test indicated that this difference was statistically significant (p = 0.0232). The Cox regression survival curves for patients infected with high MIC or low MIC isolates are shown in Figure 3. Analysis of the distribution of the SCCmec genotype and spa genotype in the high and low MIC groups indicated that most of the high-MIC isolates had the genotype SCCmec III, spa type t037 (Table 5). The high MIC group also displayed a higher resistance rate to other antibiotics (Table 5).

Figure 2

Kaplan-Meier survival curves of high-MIC and low-MIC group. Kaplan-Meier survival curves of patients with nosocomial MRSA bacteremia who were infected with high-MIC isolates (lower line) or low-MIC isolates (upper line). Log-rank test: p = 0.0232

Figure 3

Cox regression survival curves of high-MIC and low-MIC group. Cox regression survival curves of patients with nosocomial MRSA bacteremia who were infected with high-MIC isolates (lower line) or low-MIC isolates (upper line)

Table 5

Molecular and antimicrobial resistance features of high MIC and low MIC MRSA isolates

Characteristic	Vancomycin MIC < 2N = 97 Number of patients (%)	Vancomycin MIC = 2N = 26Number of patients (%)	p-valuea
SCCmec type
SCCmec II	24 (24.7)	2 (7.7)
SCCmec III	52 (53.6)	24 (92.3)
SCCmec IV	10 (10.3)
SCCmec V	11 (11.3)
Spa type
Spa t037	50 (51.5)	22 (84.6)
Spa t002	24 (24.7)	2 (7.7)
Spa t437	18 (18.6)	0 (0)
Spa t298	1 (1)	0 (0)
Spa t1081	1 (1)	0 (0)
Spa t138	0 (0)	1 (3.8)
Non-typeable	3 (3.1)	1 (3.8)
Isolates Susceptibility rate
Erythromycin	2 (2.1)	(0)	0.460
Clindamycin	14 (14.4)	1 (3.8)	0.143
Tetracycline	33 (34)	2 (7.7)	0.008
Levofloxacin	20 (20.6)	0 (0)	0.011
TMP/SMZ	45 (46.4)	2 (7.7)	< 0.001
Gentamicin	17 (13.4)	0 (0)	0.021
Rifampin	78 (80.4)	16 (61.5)	0.044

a: p-value calculated using a χ2-test

Discussion

Our results show that patients infected with isolates of MRSA with a high vancomycin MIC, as determined by standard broth microdilution methods, tend to have higher mortality. This is in agreement with previous studies using Etest as the method of susceptibility determination, which displayed differing success rates (23-45%) with vancomycin treatment of patients infected with high MIC and low MIC isolates [1-5,9]. Our survival analysis indicated that patients infected with high MIC isolates had a 16% higher mortality after 14 days and a 22% higher mortality after 30 days compared to patients infected with low MIC isolates. The poor clinical outcome of patients infected with high MIC isolates remained after adjusting for confounding variables.

Contrary to the results reported by Musta et al., our study showed patients infected with MRSA with high vancomycin MIC had a higher mortality rate, as assessed by multivariate analyses, compared to patients infected with low vancomycin MIC isolates. The reason for these differences may be explained by the use of a different MIC test (Etest vs. microdilution) and different SCCmec genotypes, i.e., SCCmec III, t037. In a previous report, vancomycin MICs generated using the Etest were consistently a one- to two-fold dilution higher than MICs determined using the CLSI broth dilution method [6,7]. We think our cohort contained more patients infected with high vancomycin MIC isolates than the Musta et al. cohort; thus, their observed difference may have been more significant if their study had involved more patients infected with high vancomycin MIC S. aureus.

The higher mortality rate of patients infected with high MIC isolates may be explained by an inadequate AUC/MIC ratio, delayed bacterial killing in the high MIC group [1-5,9], and/or the existence of strains of heteroresistant vancomycin-intermediate S. aureus [17]. The higher mortality rate in high MIC group may also be associated with other covariates that were not measured in this study such as severity of acute illness (i.e.APACHE II score) or do not resuscitate orders.

In our isolates from the high MIC group, the most common (> 80%) genotype was SCCmec III, spa type t037. MRSA sequence type (ST) 239, spa type t037, SCCmec III, is now the most prevalent nosocomial strain in many Asian countries [11,18-20] and has also been detected in 26 countries outside of Asia [21]. In a study from Singapore and Hong Kong, MRSA ST239 (SCCmec III/spa type t037) was found to comprise a higher proportion of the high vancomycin MIC isolates than other SCCmec type strains [22,23]. The distribution of staphylococcal cassette chromosome mec types and correlation with comorbidity and infection type in our patients with MRSA bacteremia has been described in another study [24]. The high vancomycin MIC and the high resistance rate to other antibiotics associated with this major clone is a therapeutic challenge for clinicians.

Broth microdilution is a standard CLSI method for determining MICs, but this technique is time consuming and labour intensive. The limitation of this study is that it is a retrospective, single center study in Taiwan and the local MRSA vancomycin MIC distribution pattern in Taiwan may not be applicable to other countries. Clonal transmission among these MRSA isolates is also possible. Additionally, other confounding factors for the mortality analysis such as more do not resuscitate order in high MIC group than low MIC group may also exist.

Conclusions

In conclusion, using a microdilution method, our study shows that patients with MRSA bacteremia who are in the ICU or who have been hospitalized for a long time may be infected with strains of high vancomycin MICs (MIC = 2 mg/L ). Patients infected with high-MIC strains had higher mortality than patients infected with low-MIC strains.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

Conceived and designed the experiments: WJL and WJT; performed the experiments: WJL and WJT; analyzed the data: WJL and WJT; wrote the paper: WJL, WJT and SWH; oversaw the running of the project: CYC and CSC. All authors have read and approved the final manuscript

Pre-publication history

The pre-publication history for this paper can be accessed here:

http://www.biomedcentral.com/1471-2334/10/159/prepub