OBJECTIVES: To determine the genetic structures at the origin of the mobilization of the extended-spectrum beta-lactamase (ESBL) bla(GES-1) gene in an Escherichia coli clinical isolate. METHODS: ESBL-encoding genes and class 1 or class 3 integron-specific motifs were screened. Conjugation experiments were performed to determine whether the plasmid-carrying bla(GES-1) gene was self-transferable. Plasmid sequencing was achieved by a primer-walking approach. RESULTS: The bla(GES-1) gene was located in a class 3 integron. That unusual genetic structure was itself located on an approximately 9 kb plasmid, pQ7, which was not self-transferable. Sequence analysis revealed that plasmid pQ7 belonged to a novel subtype of the IncQ group. CONCLUSIONS: This study identified for the first time the bla(GES-1) gene in E. coli and in Switzerland. It describes a novel IncQ-type plasmid subgroup that possesses original features, in particular iteron sequences that constitute a hot spot for integration of foreign DNA.
OBJECTIVES: To determine the genetic structures at the origin of the mobilization of the extended-spectrum beta-lactamase (ESBL) bla(GES-1) gene in an Escherichia coli clinical isolate. METHODS: ESBL-encoding genes and class 1 or class 3 integron-specific motifs were screened. Conjugation experiments were performed to determine whether the plasmid-carrying bla(GES-1) gene was self-transferable. Plasmid sequencing was achieved by a primer-walking approach. RESULTS: The bla(GES-1) gene was located in a class 3 integron. That unusual genetic structure was itself located on an approximately 9 kb plasmid, pQ7, which was not self-transferable. Sequence analysis revealed that plasmid pQ7 belonged to a novel subtype of the IncQ group. CONCLUSIONS: This study identified for the first time the bla(GES-1) gene in E. coli and in Switzerland. It describes a novel IncQ-type plasmid subgroup that possesses original features, in particular iteron sequences that constitute a hot spot for integration of foreign DNA.
Authors: Costas C Papagiannitsis; Monika Dolejska; Radoslaw Izdebski; Hana Dobiasova; Vendula Studentova; Francisco J Esteves; Lennie P G Derde; Marc J M Bonten; Jaroslav Hrabák; Marek Gniadkowski Journal: Antimicrob Agents Chemother Date: 2015-06-01 Impact factor: 5.191
Authors: Hyo Jung Lee; Hyun Mi Jin; Moon Su Park; Woojun Park; Eugene L Madsen; Che Ok Jeon Journal: Appl Environ Microbiol Date: 2014-10-10 Impact factor: 4.792