Literature DB >> 20517990

Preparation of DNA and RNA fragments containing guanine N(2)-thioalkyl tethers.

Xiaorong Hou1, Gang Wang, Barbara L Gaffney, Roger A Jones.   

Abstract

This unit describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N(2) contains a thiopropyl tether, protected as a tert-butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site-specific cross-linking to a thiol of a protein or another nucleic acid.

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Year:  2010        PMID： 20517990      PMCID： PMC2967349          DOI： 10.1002/0471142700.nc0508s41

Source DB:  PubMed          Journal:  Curr Protoc Nucleic Acid Chem        ISSN： 1934-9270


  15 in total

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Authors:  K Gao; S L Butler; F Bushman
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Review 2.  Covalent trapping of protein-DNA complexes.

Authors:  Gregory L Verdine; Derek P G Norman
Journal:  Annu Rev Biochem       Date:  2003       Impact factor: 23.643

3.  Probing the structure and function of the Escherichia coli DNA alkylation repair AlkB protein through chemical cross-linking.

Authors:  Yukiko Mishina; Chuan He
Journal:  J Am Chem Soc       Date:  2003-07-23       Impact factor: 15.419

4.  Synthesis of guanosine and deoxyguanosine phosphoramidites with cross-linkable thioalkyl tethers for direct incorporation into RNA and DNA.

Authors:  Xiaorong Hou; Gang Wang; Barbara L Gaffney; Roger A Jones
Journal:  Nucleosides Nucleotides Nucleic Acids       Date:  2009-11       Impact factor: 1.381

5.  Modifying the helical structure of DNA by design: recruitment of an architecture-specific protein to an enforced DNA bend.

Authors:  S A Wolfe; A E Ferentz; V Grantcharova; M E Churchill; G L Verdine
Journal:  Chem Biol       Date:  1995-04

6.  Improved strategies for postoligomerization synthesis of oligodeoxynucleotides bearing structurally defined adducts at the N2 position of deoxyguanosine.

Authors:  B L DeCorte; D Tsarouhtsis; S Kuchimanchi; M D Cooper; P Horton; C M Harris; T M Harris
Journal:  Chem Res Toxicol       Date:  1996 Apr-May       Impact factor: 3.739

7.  Solution structure of a DNA hairpin and its disulfide cross-linked analog.

Authors:  R J Cain; E R Zuiderweg; G D Glick
Journal:  Nucleic Acids Res       Date:  1995-06-25       Impact factor: 16.971

8.  Structures of HIV-1 RT-DNA complexes before and after incorporation of the anti-AIDS drug tenofovir.

Authors:  Steve Tuske; Stefan G Sarafianos; Arthur D Clark; Jianping Ding; Lisa K Naeger; Kirsten L White; Michael D Miller; Craig S Gibbs; Paul L Boyer; Patrick Clark; Gang Wang; Barbara L Gaffney; Roger A Jones; Donald M Jerina; Stephen H Hughes; Eddy Arnold
Journal:  Nat Struct Mol Biol       Date:  2004-04-25       Impact factor: 15.369

9.  1-(tert-butylthio)-1,2-hydrazinedicarboxylic acid derivatives. New reagents for the introduction of the S-tert-butylthio group into cysteine and cysteine derivatives.

Authors:  E Wünsch; L Moroder; S Romani
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1982-12

10.  Thermodynamic comparison of the base pairs formed by the carcinogenic lesion O6-methylguanine with reference both to Watson-Crick pairs and to mismatched pairs.

Authors:  B L Gaffney; R A Jones
Journal:  Biochemistry       Date:  1989-07-11       Impact factor: 3.162
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