| Literature DB >> 20510741 |
Kei Sato1, Chuanyi Nie, Naoko Misawa, Yuetsu Tanaka, Mamoru Ito, Yoshio Koyanagi.
Abstract
Creating a novel small animal model of HIV-1 infection that can support long-term systemic HIV-1 infection and produce HIV-1-specific immune response has a great benefit for studying HIV-1 pathogenesis in vivo. In the present study, we have generated a humanized mouse, NOG-hCD34 mouse, by transplanting newborn NOD/SCID/IL-2Rgamma(null) mice with human hematopoietic stem cells through hepatic injection. These mice were infected with a CCR5-tropic HIV-1 and were analyzed for plasma viral load, changes in peripheral blood T lymphocytes, and HIV-1-specific antibody production. High level of viral replication, increase in effector/memory CD8(+) T lymphocytes, class-switching to IgG, and production of HIV-1-specific IgGs were observed. Our findings suggest that NOG-hCD34 mice may have a wide variety of application in HIV-1 research. Copyright (c) 2009 Elsevier Ltd. All rights reserved.Entities:
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Year: 2010 PMID： 20510741 DOI： 10.1016/j.vaccine.2009.10.154
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641