Literature DB >> 20504019

Visible fluorescence detection of type III protein secretion from bacterial pathogens.

Jacob S Yount1, Lun K Tsou, Paul D Dossa, Amy L Kullas, Adrianus W M van der Velden, Howard C Hang.   

Abstract

Type III protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.

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Year:  2010        PMID： 20504019      PMCID： PMC2919334          DOI： 10.1021/ja102257v

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  14 in total

Review 1.  Protein delivery into eukaryotic cells by type III secretion machines.

Authors:  Jorge E Galán; Hans Wolf-Watz
Journal:  Nature       Date:  2006-11-30       Impact factor: 49.962

Review 2.  Salmonellae interplay with host cells.

Authors:  Andrea Haraga; Maikke B Ohlson; Samuel I Miller
Journal:  Nat Rev Microbiol       Date:  2008-01       Impact factor: 60.633

3.  A photoactivatable push-pull fluorophore for single-molecule imaging in live cells.

Authors:  Samuel J Lord; Nicholas R Conley; Hsiao-lu D Lee; Reichel Samuel; Na Liu; Robert J Twieg; W E Moerner
Journal:  J Am Chem Soc       Date:  2008-06-24       Impact factor: 15.419

4.  Virulence blockers as alternatives to antibiotics: type III secretion inhibitors against Gram-negative bacteria.

Authors:  P Keyser; M Elofsson; S Rosell; H Wolf-Watz
Journal:  J Intern Med       Date:  2008-04-03       Impact factor: 8.989

5.  Clickable fluorescent dyes for multimodal bioorthogonal imaging.

Authors:  Lun K Tsou; Mingzi M Zhang; Howard C Hang
Journal:  Org Biomol Chem       Date:  2009-10-26       Impact factor: 3.876

6.  An inhibitor of gram-negative bacterial virulence protein secretion.

Authors:  Heather B Felise; Hai V Nguyen; Richard A Pfuetzner; Kathleen C Barry; Stona R Jackson; Marie-Pierre Blanc; Philip A Bronstein; Toni Kline; Samuel I Miller
Journal:  Cell Host Microbe       Date:  2008-10-16       Impact factor: 21.023

Review 7.  Carboxypeptidase-G2-based gene-directed enzyme-prodrug therapy: a new weapon in the GDEPT armoury.

Authors:  Douglas Hedley; Lesley Ogilvie; Caroline Springer
Journal:  Nat Rev Cancer       Date:  2007-11       Impact factor: 60.716

8.  Salicylidene acylhydrazides that affect type III protein secretion in Salmonella enterica serovar typhimurium.

Authors:  Aurel Negrea; Eva Bjur; Sofia Eriksson Ygberg; Mikael Elofsson; Hans Wolf-Watz; Mikael Rhen
Journal:  Antimicrob Agents Chemother       Date:  2007-06-04       Impact factor: 5.191

9.  Inhibition of type III secretion in Salmonella enterica serovar Typhimurium by small-molecule inhibitors.

Authors:  Debra L Hudson; Abigail N Layton; Terry R Field; Alison J Bowen; Hans Wolf-Watz; Mikael Elofsson; Mark P Stevens; Edouard E Galyov
Journal:  Antimicrob Agents Chemother       Date:  2007-05-14       Impact factor: 5.191

10.  Differential activation and function of Rho GTPases during Salmonella-host cell interactions.

Authors:  Jayesh C Patel; Jorge E Galán
Journal:  J Cell Biol       Date:  2006-10-30       Impact factor: 10.539
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  7 in total

1.  S-palmitoylation and ubiquitination differentially regulate interferon-induced transmembrane protein 3 (IFITM3)-mediated resistance to influenza virus.

Authors:  Jacob S Yount; Roos A Karssemeijer; Howard C Hang
Journal:  J Biol Chem       Date:  2012-04-17       Impact factor: 5.157

2.  Epigallocatechin-3-gallate inhibits bacterial virulence and invasion of host cells.

Authors:  Lun K Tsou; Jacob S Yount; Howard C Hang
Journal:  Bioorg Med Chem       Date:  2017-03-10       Impact factor: 3.641

3.  Cytosporone B, an inhibitor of the type III secretion system of Salmonella enterica serovar Typhimurium.

Authors:  Jianfang Li; Chao Lv; Weiyang Sun; Zhenyu Li; Xiaowei Han; Yaoyao Li; Yuemao Shen
Journal:  Antimicrob Agents Chemother       Date:  2013-03-04       Impact factor: 5.191

4.  Small molecules aimed at type III secretion systems to inhibit bacterial virulence.

Authors:  Lun K Tsou; Paul D Dossa; Howard C Hang
Journal:  Medchemcomm       Date:  2013-01-01       Impact factor: 3.597

5.  Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion Substrates.

Authors:  Lun K Tsou; María Lara-Tejero; Jordan RoseFigura; Zhenrun J Zhang; Yen-Chih Wang; Jacob S Yount; Matthew Lefebre; Paul D Dossa; Junya Kato; Fulan Guan; Wing Lam; Yung-Chi Cheng; Jorge E Galán; Howard C Hang
Journal:  J Am Chem Soc       Date:  2016-02-16       Impact factor: 15.419

Review 6.  Delivery of Heterologous Proteins, Enzymes, and Antigens via the Bacterial Type III Secretion System.

Authors:  Heather A Pendergrass; Aaron E May
Journal:  Microorganisms       Date:  2020-05-21

Review 7.  Tracking Proteins Secreted by Bacteria: What's in the Toolbox?

Authors:  Benoit Maffei; Olivera Francetic; Agathe Subtil
Journal:  Front Cell Infect Microbiol       Date:  2017-05-31       Impact factor: 5.293
  7 in total

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