Literature DB >> 20499247

qPCR and mtDNA SNP analysis of experimentally degraded hair samples and its application in forensic casework.

Stephan Köhnemann1, Petra Pennekamp, Peter Fritz Schmidt, Heidi Pfeiffer.   

Abstract

A mitochondrial DNA (mtDNA) quantification PCR (qPCR) method was developed and applied in a study with experimentally degraded hair samples (first study) and in a criminal case (second study). In the first study, the amount of detectable mtDNA decreased drastically after an incubation time of 1 month on a moist tissue in a heating cabin at 37 degrees C. In the second study, when the qPCR assay showed positive quantification results, further analysis of 32 mtDNA single-nucleotide polymorphisms (SNPs) via SNaPshot technique was always possible, indicating that successful mtDNA SNP analysis of forensic samples can be guaranteed by pre-screening samples with the qPCR described here.

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Year:  2010        PMID： 20499247     DOI： 10.1007/s00414-010-0466-0

Source DB:  PubMed          Journal:  Int J Legal Med        ISSN： 0937-9827            Impact factor:   2.686


  16 in total

1.  STR typing of human telogen hairs--a new approach.

Authors:  A Hellmann; U Rohleder; H Schmitter; M Wittig
Journal:  Int J Legal Med       Date:  2001       Impact factor: 2.686

Review 2.  Forensics and mitochondrial DNA: applications, debates, and foundations.

Authors:  Bruce Budowle; Marc W Allard; Mark R Wilson; Ranajit Chakraborty
Journal:  Annu Rev Genomics Hum Genet       Date:  2003       Impact factor: 8.929

3.  Application of mtDNA SNP analysis in forensic casework.

Authors:  Stephan Köhnemann; Heidi Pfeiffer
Journal:  Forensic Sci Int Genet       Date:  2010-02-18       Impact factor: 4.882

4.  Forensic mitochondrial DNA analysis of 691 casework hairs.

Authors:  Terry Melton; Gloria Dimick; Bonnie Higgins; Lynn Lindstrom; Kimberlyn Nelson
Journal:  J Forensic Sci       Date:  2005-01       Impact factor: 1.832

5.  Mitochondrial DNA amplification success rate as a function of hair morphology.

Authors:  Katherine A Roberts; Cassandra Calloway
Journal:  J Forensic Sci       Date:  2007-01       Impact factor: 1.832

6.  'Mitominis': multiplex PCR analysis of reduced size amplicons for compound sequence analysis of the entire mtDNA control region in highly degraded samples.

Authors:  Cordula Eichmann; Walther Parson
Journal:  Int J Legal Med       Date:  2008-03-28       Impact factor: 2.686

7.  Sequence and organization of the human mitochondrial genome.

Authors:  S Anderson; A T Bankier; B G Barrell; M H de Bruijn; A R Coulson; J Drouin; I C Eperon; D P Nierlich; B A Roe; F Sanger; P H Schreier; A J Smith; R Staden; I G Young
Journal:  Nature       Date:  1981-04-09       Impact factor: 49.962

Review 8.  Human hair histogenesis for the mitochondrial DNA forensic scientist.

Authors:  C A Linch; D A Whiting; M M Holland
Journal:  J Forensic Sci       Date:  2001-07       Impact factor: 1.832

9.  Quantification of human mitochondrial DNA in a real time PCR.

Authors:  N von Wurmb-Schwark; R Higuchi; A P Fenech; C Elfstroem; C Meissner; M Oehmichen; G A Cortopassi
Journal:  Forensic Sci Int       Date:  2002-03-28       Impact factor: 2.395

10.  Chain and conformation stability of solid-state DNA: implications for room temperature storage.

Authors:  Jacques Bonnet; Marthe Colotte; Delphine Coudy; Vincent Couallier; Joseph Portier; Bénédicte Morin; Sophie Tuffet
Journal:  Nucleic Acids Res       Date:  2009-12-07       Impact factor: 16.971
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  3 in total

1.  Genetic polymorphism analysis of mitochondrial DNA from Chinese Xinjiang Kazak ethnic group by a novel mitochondrial DNA genotyping panel.

Authors:  Tong Xie; Li Hu; Yu-Xin Guo; Yu-Chun Li; Feng Chen; Bo-Feng Zhu
Journal:  Mol Biol Rep       Date:  2018-11-17       Impact factor: 2.316

2.  The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis.

Authors:  Thorsten Schwark; Patrick Meyer; Melanie Harder; Jan-Hendrick Modrow; Nicole von Wurmb-Schwark
Journal:  Transfus Med Hemother       Date:  2012-05-11       Impact factor: 3.747

3.  Genetic polymorphisms of 54 mitochondrial DNA SNP loci in Chinese Xibe ethnic minority group.

Authors:  Chun-Mei Shen; Li Hu; Chun-Hua Yang; Cai-Yong Yin; Zhi-Dan Li; Hao-Tian Meng; Yu-Xin Guo; Ting Mei; Feng Chen; Bo-Feng Zhu
Journal:  Sci Rep       Date:  2017-03-22       Impact factor: 4.379
  3 in total

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