Literature DB >> 20491471

Fluorescent ratiometric MFC probe sensitive to early stages of alpha-synuclein aggregation.

Dmytro A Yushchenko1, Jonathan A Fauerbach, Shyamala Thirunavukkuarasu, Elizabeth A Jares-Erijman, Thomas M Jovin.   

Abstract

We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of alpha-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of alpha-synuclein aggregation, a process associated with Parkinson's disease.

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Year:  2010        PMID： 20491471     DOI： 10.1021/ja102838n

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  19 in total

1.  Supramolecular non-amyloid intermediates in the early stages of α-synuclein aggregation.

Authors:  Jonathan A Fauerbach; Dmytro A Yushchenko; Sarah H Shahmoradian; Wah Chiu; Thomas M Jovin; Elizabeth A Jares-Erijman
Journal:  Biophys J       Date:  2012-03-06       Impact factor: 4.033

2.  Structural insights into amyloid oligomers of the Parkinson disease-related protein α-synuclein.

Authors:  J Ignacio Gallea; M Soledad Celej
Journal:  J Biol Chem       Date:  2014-08-20       Impact factor: 5.157

3.  Synthesis of a library of oligothiophenes and their utilization as fluorescent ligands for spectral assignment of protein aggregates.

Authors:  Therése Klingstedt; Andreas Aslund; Rozalyn A Simon; Leif B G Johansson; Jeffrey J Mason; Sofie Nyström; Per Hammarström; K Peter R Nilsson
Journal:  Org Biomol Chem       Date:  2011-11-03       Impact factor: 3.876

4.  Pre-aggregation kinetics and intermediates of α-synuclein monitored by the ESIPT probe 7MFE.

Authors:  Jonathan A Fauerbach; Thomas M Jovin
Journal:  Eur Biophys J       Date:  2017-12-18       Impact factor: 1.733

5.  Imaging nanometer-sized α-synuclein aggregates by superresolution fluorescence localization microscopy.

Authors:  M Julia Roberti; Jonas Fölling; M Soledad Celej; Mariano Bossi; Thomas M Jovin; Elizabeth A Jares-Erijman
Journal:  Biophys J       Date:  2012-04-03       Impact factor: 4.033

6.  Native chemical ligation of thioamide-containing peptides: development and application to the synthesis of labeled α-synuclein for misfolding studies.

Authors:  Solongo Batjargal; Yanxin J Wang; Jacob M Goldberg; Rebecca F Wissner; E James Petersson
Journal:  J Am Chem Soc       Date:  2012-04-02       Impact factor: 15.419

7.  Modification of C Terminus Provides New Insights into the Mechanism of α-Synuclein Aggregation.

Authors:  Kseniia Afitska; Anna Fucikova; Volodymyr V Shvadchak; Dmytro A Yushchenko
Journal:  Biophys J       Date:  2017-09-20       Impact factor: 4.033

8.  Specificity and kinetics of alpha-synuclein binding to model membranes determined with fluorescent excited state intramolecular proton transfer (ESIPT) probe.

Authors:  Volodymyr V Shvadchak; Lisandro J Falomir-Lockhart; Dmytro A Yushchenko; Thomas M Jovin
Journal:  J Biol Chem       Date:  2011-02-17       Impact factor: 5.157

9.  Residue-Specific Dynamics and Local Environmental Changes in Aβ40 Oligomer and Fibril Formation.

Authors:  Haiyang Liu; Clifford Morris; Richard Lantz; Thomas W Kent; Esmail A Elbassal; Ewa P Wojcikiewicz; Deguo Du
Journal:  Angew Chem Int Ed Engl       Date:  2018-06-14       Impact factor: 15.336

10.  Dual Functional Small Molecule Probes as Fluorophore and Ligand for Misfolding Proteins.

Authors:  Xueli Zhang; Chongzhao Ran
Journal:  Curr Org Chem       Date:  2013-03-01       Impact factor: 2.180
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