Literature DB >> 2048724

Interference of Good's buffers and other biological buffers with protein determination.

P L Lleu1, G Rebel.   

Abstract

Interference of low concentrations of Hepes and other buffers commonly used in protein determination was studied. The data show that some of these buffers interfere to differing degrees with protein determination according to the Lowry method. A study of the structure-interference relationship suggests that the group ethanolamine is involved in this interference. No interference was observed when protein was measured using bicinchonic acid at the same concentration as the Lowry reagent.

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Year:  1991        PMID： 2048724     DOI： 10.1016/0003-2697(91)90210-k

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  Differential Expression of Aquaporins in Experimental Models of Acute Lung Injury.

Authors:  Alice G Vassiliou; Nikolaos Manitsopoulos; Matina Kardara; Nikolaos A Maniatis; Stylianos E Orfanos; Anastasia Kotanidou
Journal:  In Vivo       Date:  2017 Sep-Oct       Impact factor: 2.155

2.  Poly[[{μ(3)-2-[4-(2-hy-droxy-eth-yl)piperazin-1-yl]ethane-sulfonato}-silver(I)] trihydrate].

Authors:  Stephanie M Bilinovich; Matthew J Panzner; Wiley J Youngs; Thomas C Leeper
Journal:  Acta Crystallogr Sect E Struct Rep Online       Date:  2011-08-02

3.  Induced expression and functional effects of aquaporin-1 in human leukocytes in sepsis.

Authors:  Alice G Vassiliou; Nikolaos A Maniatis; Stylianos E Orfanos; Zafeiria Mastora; Edison Jahaj; Triantafillos Paparountas; Apostolos Armaganidis; Charis Roussos; Vassilis Aidinis; Anastasia Kotanidou
Journal:  Crit Care       Date:  2013-09-12       Impact factor: 9.097

4.  Protein Determination-Method Matters.

Authors:  Hanne K Mæhre; Lars Dalheim; Guro K Edvinsen; Edel O Elvevoll; Ida-Johanne Jensen
Journal:  Foods       Date:  2018-01-01

5.  The Interplay between Aquaporin-1 and the Hypoxia-Inducible Factor 1α in a Lipopolysaccharide-Induced Lung Injury Model in Human Pulmonary Microvascular Endothelial Cells.

Authors:  Chrysi Keskinidou; Nikolaos S Lotsios; Alice G Vassiliou; Ioanna Dimopoulou; Anastasia Kotanidou; Stylianos E Orfanos
Journal:  Int J Mol Sci       Date:  2022-09-13       Impact factor: 6.208
  5 in total

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