OBJECTIVE: Because we found higher serum 25-hydroxyvitamin D (25(OH)D) levels among smokers than among non-smokers with analyses using an electrochemiluminescence immunoassay (ECLIA) from Roche, the purpose of the present study was to examine whether this difference between smokers and non-smokers was maintained using other serum 25(OH)D assays. DESIGN: A cross-sectional population-based study on 6932 participants from the Tromsø study, 1994-1995, and one validation study comparing six different serum 25(OH)D assays in 53 non-smokers and 54 smokers were performed. METHODS: The association between smoking, season and serum 25(OH)D as measured by ECLIA (Roche) was assessed in the population-based study using general linear models with multivariate adjustments. In the validation study, serum levels of 25(OH)D were analysed with liquid chromatography coupled with mass spectrometry assay from two different laboratories, RIA (DiaSorin), HPLC, RIA (IDS) and ECLIA (Roche). T-tests and linear mixed model analyses were performed to compare the serum 25(OH)D levels in smokers and non-smokers within and between the methods. RESULTS: In the population-based study, the serum levels of 25(OH)D using the ECLIA method were 51.9, 53.2 and 72.0 nmol/l in never, former and current smokers (P<0.01). In the validation study, the serum concentration of 25(OH)D was 10.3 nmol/l higher in smokers than in non-smokers (P<0.01) using the ECLIA (Roche), while non-significantly lower serum levels of 25(OH)D were found in smokers using the other five methods. CONCLUSIONS: These two studies indicate that the ECLIA (Roche) overestimates serum 25(OH)D levels in smokers by unknown mechanisms. If confirmed, this might have clinical consequences, and the issue needs further exploration.
OBJECTIVE: Because we found higher serum 25-hydroxyvitamin D (25(OH)D) levels among smokers than among non-smokers with analyses using an electrochemiluminescence immunoassay (ECLIA) from Roche, the purpose of the present study was to examine whether this difference between smokers and non-smokers was maintained using other serum 25(OH)D assays. DESIGN: A cross-sectional population-based study on 6932 participants from the Tromsø study, 1994-1995, and one validation study comparing six different serum 25(OH)D assays in 53 non-smokers and 54 smokers were performed. METHODS: The association between smoking, season and serum 25(OH)D as measured by ECLIA (Roche) was assessed in the population-based study using general linear models with multivariate adjustments. In the validation study, serum levels of 25(OH)D were analysed with liquid chromatography coupled with mass spectrometry assay from two different laboratories, RIA (DiaSorin), HPLC, RIA (IDS) and ECLIA (Roche). T-tests and linear mixed model analyses were performed to compare the serum 25(OH)D levels in smokers and non-smokers within and between the methods. RESULTS: In the population-based study, the serum levels of 25(OH)D using the ECLIA method were 51.9, 53.2 and 72.0 nmol/l in never, former and current smokers (P<0.01). In the validation study, the serum concentration of 25(OH)D was 10.3 nmol/l higher in smokers than in non-smokers (P<0.01) using the ECLIA (Roche), while non-significantly lower serum levels of 25(OH)D were found in smokers using the other five methods. CONCLUSIONS: These two studies indicate that the ECLIA (Roche) overestimates serum 25(OH)D levels in smokers by unknown mechanisms. If confirmed, this might have clinical consequences, and the issue needs further exploration.
Authors: José Manuel Ordóñez-Mena; Ben Schöttker; Veronika Fedirko; Mazda Jenab; Anja Olsen; Jytte Halkjær; Ellen Kampman; Lisette de Groot; Eugene Jansen; H Bas Bueno-de-Mesquita; Petra H Peeters; Galatios Siganos; Tom Wilsgaard; Laura Perna; Bernd Holleczek; Ulrika Pettersson-Kymmer; Philippos Orfanos; Antonia Trichopoulou; Paolo Boffetta; Hermann Brenner Journal: Eur J Epidemiol Date: 2015-05-15 Impact factor: 8.082
Authors: Lu Wang; Yiqing Song; Joann E Manson; Stefan Pilz; Winfried März; Karl Michaëlsson; Annamari Lundqvist; Simerjot K Jassal; Elizabeth Barrett-Connor; Cuilin Zhang; Charles B Eaton; Heidi T May; Jeffrey L Anderson; Howard D Sesso Journal: Circ Cardiovasc Qual Outcomes Date: 2012-11-13
Authors: Gaffar Sarwar Zaman; Safar Abadi Saeed Al-Saleem Alshahrani; Nasrin Banu Laskar; Ibrahim Hadadi; Magbool Alelyani; Mohamed Adam; Mohammed Babiker; Mustafa Jafar Musa; Pranab Barua; Mohammed Elimam Ahamed Mohammed Journal: Int J Gen Med Date: 2022-02-07
Authors: Dennis Back Holmgaard; Lone Hagens Mygind; Ingrid Louise Titlestad; Hanne Madsen; Palle Bach Nielsen Fruekilde; Svend Stenvang Pedersen; C Pedersen Journal: PLoS One Date: 2013-01-14 Impact factor: 3.240