| Literature DB >> 20473289 |
Dong-Uk Kim1, Jacqueline Hayles2, Dongsup Kim3, Valerie Wood2,4, Han-Oh Park5, Misun Won1, Hyang-Sook Yoo1, Trevor Duhig2, Miyoung Nam1, Georgia Palmer2, Sangjo Han3, Linda Jeffery2, Seung-Tae Baek1, Hyemi Lee1, Young Sam Shim1, Minho Lee3, Lila Kim1, Kyung-Sun Heo1, Eun Joo Noh1, Ah-Reum Lee1, Young-Joo Jang1, Kyung-Sook Chung1, Shin-Jung Choi1, Jo-Young Park1, Youngwoo Park1, Hwan Mook Kim6, Song-Kyu Park6, Hae-Joon Park5, Eun-Jung Kang5, Hyong Bai Kim7, Hyun-Sam Kang8, Hee-Moon Park9, Kyunghoon Kim10, Kiwon Song11, Kyung Bin Song12, Paul Nurse2,13, Kwang-Lae Hoe1,6.
Abstract
We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast--the only other eukaryote for which a comprehensive knockout library exists--revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.Entities:
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Year: 2010 PMID： 20473289 PMCID： PMC3962850 DOI： 10.1038/nbt.1628
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908