| Literature DB >> 20470754 |
Guojun Cao1, Baochun Huang, Zhaohui Liu, Jiyan Zhang, Hua Xu, Wei Xia, Jie Li, Shaohua Li, Liucun Chen, Hongmei Ding, Qiang Zhao, Ming Fan, Beifen Shen, Ningsheng Shao.
Abstract
miR-301 is localized in the first intron of ska2, whose function has not been clarified. Here, a new circuit model in which intronic miR-301 regulates the transcription and function of its host gene through a feedback mechanism has been described. Our results showed that blocking of miR-301 in A549 cells leads to a decrease in the expression of the host gene, ska2. Further analysis showed that miR-301 targets MEOX2 to affect the ERK/CREB pathway. CREB directly regulates the expression of the host gene, ska2. In addition, the inhibition of miR-301 or ska2 resulted in an increase of the mitotic index and a decrease in colony formation in soft agar, which may be related to lung tumorigenesis. (c) 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20470754 DOI： 10.1016/j.bbrc.2010.05.037
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575