OBJECTIVE: To describe a case of severe and drug-resistant Acanthamoeba keratitis in a contact lens wearer caused by atypical T5 Acanthamoeba genotype (Acanthamoeba lenticulata). METHODS: Report of a case, Acanthamoeba DNA amplification and sequencing. RESULTS: A 61-year-old patient was referred to our clinic with a 2-week history of keratitis. Acanthamoeba keratitis (AK) was diagnosed using confocal microscopy and corneal scraping culture. Using polymerase chain reaction (PCR) and DNA sequencing, the organism was classified as a T5 genotype (A. lenticulata). The keratitis continued to progress despite topical antiamoebic therapy and ultimately led to enucleation of the affected eye. CONCLUSIONS: T5 genotype Acanthamoeba can cause severe AK. Atypical Acanthamoeba genotypes could be associated with worse prognosis and resistance to therapy.
OBJECTIVE: To describe a case of severe and drug-resistant Acanthamoeba keratitis in a contact lens wearer caused by atypical T5 Acanthamoeba genotype (Acanthamoeba lenticulata). METHODS: Report of a case, Acanthamoeba DNA amplification and sequencing. RESULTS: A 61-year-old patient was referred to our clinic with a 2-week history of keratitis. Acanthamoeba keratitis (AK) was diagnosed using confocal microscopy and corneal scraping culture. Using polymerase chain reaction (PCR) and DNA sequencing, the organism was classified as a T5 genotype (A. lenticulata). The keratitis continued to progress despite topical antiamoebic therapy and ultimately led to enucleation of the affected eye. CONCLUSIONS: T5 genotype Acanthamoeba can cause severe AK. Atypical Acanthamoeba genotypes could be associated with worse prognosis and resistance to therapy.
Authors: Francisco Arnalich-Montiel; Blanca Lumbreras-Fernández; Carmen M Martín-Navarro; Basilio Valladares; Rogelio Lopez-Velez; Rafael Morcillo-Laiz; Jacob Lorenzo-Morales Journal: J Clin Microbiol Date: 2014-01-15 Impact factor: 5.948
Authors: T Martín-Pérez; T Lozano-Cruz; A Criado-Fornelio; P Ortega; R Gómez; F J de la Mata; J Pérez-Serrano Journal: Parasitol Res Date: 2019-05-08 Impact factor: 2.289
Authors: Ute Scheikl; Regina Sommer; Alexander Kirschner; Alexandra Rameder; Barbara Schrammel; Irene Zweimüller; Wolfgang Wesner; Manfred Hinker; Julia Walochnik Journal: Eur J Protistol Date: 2014-05-13 Impact factor: 3.020