BACKGROUND: In chronic renal failure (CRF), residual nephrons can increase their excretion of sodium (Na) and potassium (K). However, the mechanisms of renal Na and K regulation in late-stage CRF have not been clearly investigated. METHODS: We examined altered expression of major renal Na and K transporters in Sprague-Dawley rats at 4 and 12 weeks after a 5/6 nephrectomy. RESULTS: CRF rats were azotemic and had gradually increased levels of urinary Na and K excretion over time. At 4 weeks, the abundance of Na-K-2Cl cotransporter (NKCC2), and Na-Cl cotransporter (NCC) in CRF rats increased significantly (477 and 222% of the control, respectively). In contrast, expression of NKCC2 and NCC decreased markedly at 12 weeks (55.4 and 30.8%, respectively). Expression of epithelial Na channel-alpha increased throughout the whole period. The abundance of renal outer medullary K-channel (ROMK) and Na-K-ATPase did not decrease at 4 weeks, but it was reduced at 12 weeks. CONCLUSION: We suggest that increased urinary Na excretion in late-stage CRF may be associated with decreased expression of renal Na transporters except ENaC compared to early-stage CRF, and that increased urinary K excretion in the late stage of CRF may not be related to expression of ROMK. Copyright 2010 S. Karger AG, Basel.
BACKGROUND: In chronic renal failure (CRF), residual nephrons can increase their excretion of sodium (Na) and potassium (K). However, the mechanisms of renal Na and K regulation in late-stage CRF have not been clearly investigated. METHODS: We examined altered expression of major renal Na and K transporters in Sprague-Dawley rats at 4 and 12 weeks after a 5/6 nephrectomy. RESULTS: CRF rats were azotemic and had gradually increased levels of urinary Na and K excretion over time. At 4 weeks, the abundance of Na-K-2Cl cotransporter (NKCC2), and Na-Cl cotransporter (NCC) in CRF rats increased significantly (477 and 222% of the control, respectively). In contrast, expression of NKCC2 and NCC decreased markedly at 12 weeks (55.4 and 30.8%, respectively). Expression of epithelial Na channel-alpha increased throughout the whole period. The abundance of renal outer medullary K-channel (ROMK) and Na-K-ATPase did not decrease at 4 weeks, but it was reduced at 12 weeks. CONCLUSION: We suggest that increased urinary Na excretion in late-stage CRF may be associated with decreased expression of renal Na transporters except ENaC compared to early-stage CRF, and that increased urinary K excretion in the late stage of CRF may not be related to expression of ROMK. Copyright 2010 S. Karger AG, Basel.
Authors: Dominique M Bovée; Wesley J Visser; Igor Middel; Anneke De Mik-van Egmond; Rick Greupink; Rosalinde Masereeuw; Frans G M Russel; A H Jan Danser; Robert Zietse; Ewout J Hoorn Journal: J Am Soc Nephrol Date: 2020-01-29 Impact factor: 10.121
Authors: Sejoong Kim; Jin Young Yang; Eun Sook Jung; Jeonghwan Lee; Nam Ju Heo; Jae Wook Lee; Ki Young Na; Jin Suk Han Journal: J Korean Med Sci Date: 2014-11-21 Impact factor: 2.153
Authors: Sejoong Kim; Eun Sook Jung; Jeonghwan Lee; Nam Ju Heo; Ki Young Na; Jin Suk Han Journal: Korean J Intern Med Date: 2017-03-18 Impact factor: 2.884