Literature DB >> 20453135

Cloning and characterization of a gene cluster for hatomarubigin biosynthesis in Streptomyces sp. strain 2238-SVT4.

Takashi Kawasaki1, Reiko Hirashima, Tomoka Maruta, Haruka Sato, Ayumi Maeda, Yuki Yamada, Maho Takeda, Yoichi Hayakawa.   

Abstract

Streptomyces sp. strain 2238-SVT4 produces hatomarubigins A, B, C, and D, which belong to the angucycline family. Among them, hatomarubigin D has a unique dimeric structure with a methylene linkage. PCR using aromatase and cyclase gene-specific primers identified the hrb gene cluster for angucycline biosynthesis in Streptomyces sp. 2238-SVT4. The cluster consisted of 30 open reading frames, including those for the minimal polyketide synthase, ketoreductase, aromatase, cyclase, O-methyltransferase, oxidoreductase, and oxygenase genes. Expression of a part of the gene cluster containing hrbR1 to hrbX in Streptomyces lividans TK23 resulted in the production of hatomarubigins A, B, and C. Hatomarubigin D was obtained from the conversion of hatomarubigin C by a purified enzyme encoded by hrbY, among the remaining genes.

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Year:  2010        PMID： 20453135      PMCID： PMC2897453          DOI： 10.1128/AEM.00668-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Two new tailoring enzymes, a glycosyltransferase and an oxygenase, involved in biosynthesis of the angucycline antibiotic urdamycin A in Streptomyces fradiae Tü2717.

Authors:  B Faust; D Hoffmeister; G Weitnauer; L Westrich; S Haag; P Schneider; H Decker; E Künzel; J Rohr; A Bechthold
Journal:  Microbiology       Date:  2000-01       Impact factor: 2.777

2.  Autoregulation of antibiotic biosynthesis by binding of the end product to an atypical response regulator.

Authors:  Linqi Wang; Xiuyun Tian; Juan Wang; Haihua Yang; Keqiang Fan; Gangming Xu; Keqian Yang; Huarong Tan
Journal:  Proc Natl Acad Sci U S A       Date:  2009-05-07       Impact factor: 11.205

3.  Functional analyses of oxygenases in jadomycin biosynthesis and identification of JadH as a bifunctional oxygenase/dehydrase.

Authors:  Yi-Hua Chen; Chen-Chen Wang; Lisa Greenwell; Uwe Rix; Dirk Hoffmeister; Leo C Vining; Jürgen Rohr; Ke-Qian Yang
Journal:  J Biol Chem       Date:  2005-04-06       Impact factor: 5.157

4.  Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis.

Authors:  L Westrich; S Domann; B Faust; D Bedford; D A Hopwood; A Bechthold
Journal:  FEMS Microbiol Lett       Date:  1999-01-15       Impact factor: 2.742

5.  Identification of the function of gene lndM2 encoding a bifunctional oxygenase-reductase involved in the biosynthesis of the antitumor antibiotic landomycin E by Streptomyces globisporus 1912 supports the originally assigned structure for landomycinone.

Authors:  Lili Zhu; Bohdan Ostash; Uwe Rix; Mohammad Nur-E-Alam; Almuth Mayers; Andriy Luzhetskyy; Carmen Mendez; Jose A Salas; Andreas Bechthold; Victor Fedorenko; Jürgen Rohr
Journal:  J Org Chem       Date:  2005-01-21       Impact factor: 4.354

6.  The tcmVI region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens encodes the tetracenomycin F1 monooxygenase, tetracenomycin F2 cyclase, and, most likely, a second cyclase.

Authors:  R G Summers; E Wendt-Pienkowski; H Motamedi; C R Hutchinson
Journal:  J Bacteriol       Date:  1993-12       Impact factor: 3.490

7.  LanV, a bifunctional enzyme: aromatase and ketoreductase during landomycin A biosynthesis.

Authors:  Almuth Mayer; Takaaki Taguchi; Anton Linnenbrink; Carsten Hofmann; Andriy Luzhetskyy; Andreas Bechthold
Journal:  Chembiochem       Date:  2005-12       Impact factor: 3.164

Review 8.  Sequence-structure analysis of FAD-containing proteins.

Authors:  O Dym; D Eisenberg
Journal:  Protein Sci       Date:  2001-09       Impact factor: 6.725

9.  Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230.

Authors:  L Han; K Yang; E Ramalingam; R H Mosher; L C Vining
Journal:  Microbiology       Date:  1994-12       Impact factor: 2.777

10.  Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338.

Authors:  Markiyan Oliynyk; Markiyan Samborskyy; John B Lester; Tatiana Mironenko; Nataliya Scott; Shilo Dickens; Stephen F Haydock; Peter F Leadlay
Journal:  Nat Biotechnol       Date:  2007-03-18       Impact factor: 54.908
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  2 in total

Review 1.  The functional differentiation of the post-PKS tailoring oxygenases contributed to the chemical diversities of atypical angucyclines.

Authors:  Keqiang Fan; Qian Zhang
Journal:  Synth Syst Biotechnol       Date:  2018-11-14

2.  Genome mining of novel rubiginones from Streptomyces sp. CB02414 and characterization of the post-PKS modification steps in rubiginone biosynthesis.

Authors:  Jingyan Zhang; Ying Sun; Yeji Wang; Xin Chen; Lu Xue; Jingjing Zhang; Xiangcheng Zhu; Yanwen Duan; Xiaohui Yan
Journal:  Microb Cell Fact       Date:  2021-10-02       Impact factor: 5.328
  2 in total

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