UNLABELLED: ETHNOPHARMACOLOGYCAL RELEVANCE: The tea from the leaves of Baccharis illinita DC (Asteraceae family) is commonly used by the population as anti-inflammatory (including topically), protective gastric and anti-infectious. However, no studies have been done with this species to confirm its topical anti-inflammatory action. AIM: This study evaluated he topical effects of crude extract of leaves (CE) and its active constituents in 12-O-tetradecanoylphorbol acetate (TPA)-induced ear oedema. METHODOLOGY: CE and compounds effects were tested in commonly used models of TPA-, arachidonic acid (AA)- and capsaicin-ear oedema. Polymorphonuclear (PMN) cell migration was evaluated by mieloperoxidase and analyzed histologically. RESULTS: CE (0.1-1 mg/ear) caused a dose-related inhibition of TPA-induced ear oedema and PMN influx similarly to that produced by topical application of the steroidal anti-inflammatory drug dexamethasone. The active constituents of the AcOEt fraction kaurenoic acid, alpha-spinasterol, oleanolic acid and baurenol also inhibited TPA-induced ear edema. Histological analysis of the ear of CE-treated animals confirmed the reduction of edema and of PMN infiltration. Both CE and the nosteroidal anti-inflammatory drug indomethacin inhibited the AA-induced ear oedema, but did not change capsaicin-induced oedema. CONCLUSION: These results indicate that the CE and the active constituents have a topical anti-inflammatory effect and the possible mechanisms for the pharmacological effects are discussed. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
UNLABELLED: ETHNOPHARMACOLOGYCAL RELEVANCE: The tea from the leaves of Baccharis illinita DC (Asteraceae family) is commonly used by the population as anti-inflammatory (including topically), protective gastric and anti-infectious. However, no studies have been done with this species to confirm its topical anti-inflammatory action. AIM: This study evaluated he topical effects of crude extract of leaves (CE) and its active constituents in 12-O-tetradecanoylphorbol acetate (TPA)-induced ear oedema. METHODOLOGY: CE and compounds effects were tested in commonly used models of TPA-, arachidonic acid (AA)- and capsaicin-ear oedema. Polymorphonuclear (PMN) cell migration was evaluated by mieloperoxidase and analyzed histologically. RESULTS: CE (0.1-1 mg/ear) caused a dose-related inhibition of TPA-induced ear oedema and PMN influx similarly to that produced by topical application of the steroidal anti-inflammatory drug dexamethasone. The active constituents of the AcOEt fraction kaurenoic acid, alpha-spinasterol, oleanolic acid and baurenol also inhibited TPA-induced ear edema. Histological analysis of the ear of CE-treated animals confirmed the reduction of edema and of PMN infiltration. Both CE and the nosteroidal anti-inflammatory drug indomethacin inhibited the AA-induced ear oedema, but did not change capsaicin-induced oedema. CONCLUSION: These results indicate that the CE and the active constituents have a topical anti-inflammatory effect and the possible mechanisms for the pharmacological effects are discussed. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Authors: Mirele da Silveira Vasconcelos; Neuza F Gomes-Rochette; Maria Liduína M de Oliveira; Diana Célia S Nunes-Pinheiro; Adriana R Tomé; Francisco Yuri Maia de Sousa; Francisco Geraldo M Pinheiro; Carlos Farley H Moura; Maria Raquel A Miranda; Erika Freitas Mota; Dirce Fernandes de Melo Journal: Exp Biol Med (Maywood) Date: 2015-03-27
Authors: Luiz Felipe D Passero; Alexis Bonfim-Melo; Carlos Eduardo P Corbett; Márcia D Laurenti; Marcos H Toyama; Daniela O de Toyama; Paulete Romoff; Oriana A Fávero; Simone S dos Grecco; Cynthia A Zalewsky; João Henrique G Lago Journal: Parasitol Res Date: 2010-10-01 Impact factor: 2.289