Literature DB >> 20449833

Single point vs. mapping approach for spectral cytopathology (SCP).

Jennifer M Schubert1, Antonella I Mazur, Benjamin Bird, Milos Miljković, Max Diem.   

Abstract

In this paper we describe the advantages of collecting infrared microspectral data in imaging mode opposed to point mode. Imaging data are processed using the PapMap algorithm, which co-adds pixel spectra that have been scrutinized for R-Mie scattering effects as well as other constraints. The signal-to-noise quality of PapMap spectra will be compared to point spectra for oral mucosa cells deposited onto low-e slides. Also the effects of software atmospheric correction will be discussed. Combined with the PapMap algorithm, data collection in imaging mode proves to be a superior method for spectral cytopathology. (c) 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2010        PMID： 20449833      PMCID： PMC3076557          DOI： 10.1002/jbio.201000023

Source DB:  PubMed          Journal:  J Biophotonics        ISSN： 1864-063X            Impact factor:   3.207


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