PURPOSE: This study describes a quantitative method to estimate the migratory capacity of neural stem cells (NSCs) using magnetic resonance imaging. PROCEDURES: NSCs were labeled with ferumoxides and injected stereotaxically into the corpus callosum of the normal rat brain. Control subjects received either free ferumoxides or nonviable labeled cells. Subjects were scanned after initial injection and at 1 week. Image sets were coregistered, compared morphologically, and analyzed parametrically to determine migration speed. RESULTS: Subjects receiving injections of viable cells had a significantly greater spread of the tracer after 1 week than either control group (p< 0.05). The speed of migration for viable NSCs was significantly higher than that of controls along the corpus callosum (p < 0.05). Migratory speeds estimated from histology and imaging were significantly correlated. CONCLUSIONS: This study provides a quantitative assessment of posttransplantation neural stem cell migration that is clearly distinguishable from tracer clearance.
PURPOSE: This study describes a quantitative method to estimate the migratory capacity of neural stem cells (NSCs) using magnetic resonance imaging. PROCEDURES: NSCs were labeled with ferumoxides and injected stereotaxically into the corpus callosum of the normal rat brain. Control subjects received either free ferumoxides or nonviable labeled cells. Subjects were scanned after initial injection and at 1 week. Image sets were coregistered, compared morphologically, and analyzed parametrically to determine migration speed. RESULTS: Subjects receiving injections of viable cells had a significantly greater spread of the tracer after 1 week than either control group (p< 0.05). The speed of migration for viable NSCs was significantly higher than that of controls along the corpus callosum (p < 0.05). Migratory speeds estimated from histology and imaging were significantly correlated. CONCLUSIONS: This study provides a quantitative assessment of posttransplantation neural stem cell migration that is clearly distinguishable from tracer clearance.
Authors: Raphael Guzman; Nobuko Uchida; Tonya M Bliss; Dongping He; Karen K Christopherson; David Stellwagen; Alexandra Capela; Joan Greve; Robert C Malenka; Michael E Moseley; Theo D Palmer; Gary K Steinberg Journal: Proc Natl Acad Sci U S A Date: 2007-06-06 Impact factor: 11.205
Authors: Jennifer D Newcomb; Craig T Ajmo; Cyndy D Sanberg; Paul R Sanberg; Keith R Pennypacker; Alison E Willing Journal: Cell Transplant Date: 2006 Impact factor: 4.064
Authors: Ali S Arbab; Lindsey A Bashaw; Bradley R Miller; Elaine K Jordan; Bobbi K Lewis; Heather Kalish; Joseph A Frank Journal: Radiology Date: 2003-12 Impact factor: 11.105
Authors: Matthew F McManus; Ilya M Nasrallah; Pallavi P Gopal; William S Baek; Jeffrey A Golden Journal: J Neuropathol Exp Neurol Date: 2004-09 Impact factor: 3.685
Authors: Zheng Gang Zhang; Quan Jiang; Ruilan Zhang; Li Zhang; Lei Wang; Lijei Zhang; Polly Arniego; Khang-Loon Ho; Michael Chopp Journal: Ann Neurol Date: 2003-02 Impact factor: 10.422
Authors: Joshua A Wood; Dai-Jung Chung; Shin Ae Park; Allison L Zwingenberger; Christopher M Reilly; Irene Ly; Naomi J Walker; William Vernau; Kei Hayashi; Erik R Wisner; Matthew S Cannon; Philip H Kass; Simon R Cherry; Dori L Borjesson; Paul Russell; Christopher J Murphy Journal: J Ocul Pharmacol Ther Date: 2011-12-16 Impact factor: 2.671
Authors: Henry Ruiz-Garcia; Keila Alvarado-Estrada; Sunil Krishnan; Alfredo Quinones-Hinojosa; Daniel M Trifiletti Journal: Front Bioeng Biotechnol Date: 2020-12-07