OBJECTIVE: To evaluate the effects of low-level helium-neon (HeNe) laser on cutaneous wound healing in rats. METHODS: Sixty Wistar rats were divided into control group and experimental group. A sutured longitudinal, dorsal-medial incision was made, with simple separate stitches. The experimental group was irradiated daily in three areas of the wound with HeNe laser (5mW maximum continuous power, 632.8 nm wavelength, 4 J/cm(2) energy density and 0.015 cm(2) laser beam area) for 36 seconds. The areas were evaluated on the third, seventh and fourteenth days postoperative. Histological sections were stained with hematoxylin-eosin to determine the type of inflammatory reaction according to the protocol by Vizzotto et al. (2003)* and with Picrosirius to identify types I and III collagen and the collagen maturation index (CMI). Immunohistochemical detection was employed with anti-CD45-LCA to recognize the inflammatory cells. RESULTS: Both groups had the same inflammatory pattern. The experimental group had fewer inflammatory cells at the three evaluation times (p<0.001) with faster reduction in the number of leukocytes. The experimental group had greater total collagen density on the third day (p=0.001), with more type III collagen (p=0.001) and more type I collagen (p=0.001). There was no significant difference in the CMI. CONCLUSION: Low-level laser irradiation does not change the quality of the inflammatory response, but it does reduce its intensity; it increases collagen deposition in the early stages of the healing process and does not interfere with collagen maturation.
OBJECTIVE: To evaluate the effects of low-level helium-neon (HeNe) laser on cutaneous wound healing in rats. METHODS: Sixty Wistar rats were divided into control group and experimental group. A sutured longitudinal, dorsal-medial incision was made, with simple separate stitches. The experimental group was irradiated daily in three areas of the wound with HeNe laser (5mW maximum continuous power, 632.8 nm wavelength, 4 J/cm(2) energy density and 0.015 cm(2) laser beam area) for 36 seconds. The areas were evaluated on the third, seventh and fourteenth days postoperative. Histological sections were stained with hematoxylin-eosin to determine the type of inflammatory reaction according to the protocol by Vizzotto et al. (2003)* and with Picrosirius to identify types I and III collagen and the collagen maturation index (CMI). Immunohistochemical detection was employed with anti-CD45-LCA to recognize the inflammatory cells. RESULTS: Both groups had the same inflammatory pattern. The experimental group had fewer inflammatory cells at the three evaluation times (p<0.001) with faster reduction in the number of leukocytes. The experimental group had greater total collagen density on the third day (p=0.001), with more type III collagen (p=0.001) and more type I collagen (p=0.001). There was no significant difference in the CMI. CONCLUSION: Low-level laser irradiation does not change the quality of the inflammatory response, but it does reduce its intensity; it increases collagen deposition in the early stages of the healing process and does not interfere with collagen maturation.
Authors: Cristiano S Baldan; Igor Fb Masson; Ivaldo Esteves Júnior; Alessandra Maria S Baldan; Aline F P Machado; Raquel A Casaroto; Richard E Liebano Journal: Plast Surg (Oakv) Date: 2015 Impact factor: 0.947
Authors: Franciane Barbieri Fiorio; Solange Almeida Dos Santos; Caroline Sobral de Melo Rambo; Camila Guerra Dalbosco; Andrey Jorge Serra; Brunno Lemes de Melo; Ernesto Cesar Pinto Leal-Junior; Paulo de Tarso Camillo de Carvalho Journal: Lasers Med Sci Date: 2017-07-05 Impact factor: 3.161
Authors: C C S Martignago; R F Oliveira; D A A Pires-Oliveira; P D Oliveira; C Pacheco Soares; P S Monzani; R C Poli-Frederico Journal: Lasers Med Sci Date: 2014-08-30 Impact factor: 3.161