OBJECTIVE: The present study was conducted to investigate the effects of helium-neon (He-Ne) laser irradiation on the proliferation, migration, and differentiation of cultured human epidermal stem cells (ESCs). BACKGROUND DATA: A He-Ne laser with a wavelength of 632.8 nm is known to have photobiological effects, and is widely used for accelerating wound healing; however, the cellular mechanisms involved have not been completely understood. METHODS: The ESCs were prepared from human foreskin, and irradiated by using He-Ne laser at 632.8 nm with 2 J/cm(2). The ESC proliferation, migration, and differentiation were examined by using XTT assay, scratch assay, and flow cytometry technology, respectively. The phosphorylation of extracellular signal-regulated kinases (ERK) was analyzed by using Western blotting. RESULTS: He-Ne laser irradiation markedly promoted cell proliferation and migration accompanied by an increase in the phosphorylation of ERK, but did not significantly influence cell differentiation. CONCLUSION: Our data indicated that photostimulation with a He-Ne laser resulted in a significant increase in human ESC proliferation and migration in vitro, which might contribute, at least partially, to accelerated wound re-epithelialization by low-level laser therapy.
OBJECTIVE: The present study was conducted to investigate the effects of helium-neon (He-Ne) laser irradiation on the proliferation, migration, and differentiation of cultured human epidermal stem cells (ESCs). BACKGROUND DATA: A He-Ne laser with a wavelength of 632.8 nm is known to have photobiological effects, and is widely used for accelerating wound healing; however, the cellular mechanisms involved have not been completely understood. METHODS: The ESCs were prepared from human foreskin, and irradiated by using He-Ne laser at 632.8 nm with 2 J/cm(2). The ESC proliferation, migration, and differentiation were examined by using XTT assay, scratch assay, and flow cytometry technology, respectively. The phosphorylation of extracellular signal-regulated kinases (ERK) was analyzed by using Western blotting. RESULTS: He-Ne laser irradiation markedly promoted cell proliferation and migration accompanied by an increase in the phosphorylation of ERK, but did not significantly influence cell differentiation. CONCLUSION: Our data indicated that photostimulation with a He-Ne laser resulted in a significant increase in human ESC proliferation and migration in vitro, which might contribute, at least partially, to accelerated wound re-epithelialization by low-level laser therapy.
Authors: Albert Amat; Josepa Rigau; Ronald W Waynant; Ilko K Ilev; Juanita J Anders Journal: J Photochem Photobiol B Date: 2005-11-21 Impact factor: 6.252
Authors: Cíntia Helena Santuzzi; Hygor Franca Buss; Diego França Pedrosa; Martha Oliveira Vieira Moniz Freire; Breno Valentim Nogueira; Washington Luiz Silva Gonçalves Journal: An Bras Dermatol Date: 2011 Mar-Apr Impact factor: 1.896
Authors: Khalid M AlGhamdi; Ashok Kumar; Attieh A Al-Ghamdi; Ammar C Al-Rikabi; Mohammed Mubarek; Abdelkader E Ashour Journal: Lasers Med Sci Date: 2016-08-30 Impact factor: 3.161