Literature DB >> 20409815

Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii.

Carlo Iomini1, Jacob E Till, Susan K Dutcher.   

Abstract

Conditional mutants for flagellar assembly (fla) provide a useful tool to study intraflagellar transport (IFT) at the molecular level, and provide a unique set of tools to analyze cilia. The analysis of IFT phenotypes of fla mutants at the permissive temperature by a quantitative image analysis approach identified four distinct phases of the IFT cycle and directly demonstrated structural and functional remodeling of IFT particles at both axonemal extremities. In addition, the genetic analysis of fla mutants reveal interesting interactions among genes involved in flagellar assembly that help to provide information about the structure and function of IFT particles and their motors. This chapter provides protocols to isolate, characterize, and identify conditional Chlamydomonas flagellar assembly mutants and their genes and to test genetic interactions among proteins encoded by these genes. 2009 Elsevier Inc. All rights reserved.

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Year:  2009        PMID： 20409815      PMCID： PMC4090777          DOI： 10.1016/S0091-679X(08)93007-7

Source DB:  PubMed          Journal:  Methods Cell Biol        ISSN： 0091-679X            Impact factor:   1.441


  43 in total

1.  Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.

Authors:  D G Cole; D R Diener; A L Himelblau; P L Beech; J C Fuster; J L Rosenbaum
Journal:  J Cell Biol       Date:  1998-05-18       Impact factor: 10.539

2.  Extensive restriction fragment length polymorphisms in a new isolate of Chlamydomonas reinhardtii.

Authors:  C H Gross; L P Ranum; P A Lefebvre
Journal:  Curr Genet       Date:  1988-06       Impact factor: 3.886

3.  High-efficiency transformation of Chlamydomonas reinhardtii by electroporation.

Authors:  K Shimogawara; S Fujiwara; A Grossman; H Usuda
Journal:  Genetics       Date:  1998-04       Impact factor: 4.562

4.  Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella.

Authors:  G Piperno; K Mead
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-29       Impact factor: 11.205

5.  The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes delta-tubulin, a new member of the tubulin superfamily.

Authors:  S K Dutcher; E C Trabuco
Journal:  Mol Biol Cell       Date:  1998-06       Impact factor: 4.138

6.  Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects.

Authors:  G Piperno; E Siuda; S Henderson; M Segil; H Vaananen; M Sassaroli
Journal:  J Cell Biol       Date:  1998-12-14       Impact factor: 10.539

7.  Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella.

Authors:  G J Pazour; B L Dickert; Y Vucica; E S Seeley; J L Rosenbaum; G B Witman; D G Cole
Journal:  J Cell Biol       Date:  2000-10-30       Impact factor: 10.539

8.  Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella.

Authors:  Yuqing Hou; Hongmin Qin; John A Follit; Gregory J Pazour; Joel L Rosenbaum; George B Witman
Journal:  J Cell Biol       Date:  2007-02-20       Impact factor: 10.539

9.  Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1(FLA10) to reach the distal part of flagella in Chlamydomonas.

Authors:  G Piperno; K Mead; S Henderson
Journal:  J Cell Biol       Date:  1996-04       Impact factor: 10.539

10.  The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly.

Authors:  G J Pazour; B L Dickert; G B Witman
Journal:  J Cell Biol       Date:  1999-02-08       Impact factor: 10.539
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  10 in total

1.  In situ localization of N and C termini of subunits of the flagellar nexin-dynein regulatory complex (N-DRC) using SNAP tag and cryo-electron tomography.

Authors:  Kangkang Song; Junya Awata; Douglas Tritschler; Raqual Bower; George B Witman; Mary E Porter; Daniela Nicastro
Journal:  J Biol Chem       Date:  2015-01-06       Impact factor: 5.157

2.  The Joubert syndrome protein ARL13B binds tubulin to maintain uniform distribution of proteins along the ciliary membrane.

Authors:  Ekaterina Revenkova; Qing Liu; G Luca Gusella; Carlo Iomini
Journal:  J Cell Sci       Date:  2018-05-04       Impact factor: 5.285

3.  Structural organization of the C1b projection within the ciliary central apparatus.

Authors:  Kai Cai; Yanhe Zhao; Lei Zhao; Nhan Phan; Yuqing Hou; Xi Cheng; George B Witman; Daniela Nicastro
Journal:  J Cell Sci       Date:  2021-11-10       Impact factor: 5.285

4.  Components of Intraflagellar Transport Complex A Function Independently of the Cilium to Regulate Canonical Wnt Signaling in Drosophila.

Authors:  Sophie Balmer; Aurore Dussert; Giovanna M Collu; Elvira Benitez; Carlo Iomini; Marek Mlodzik
Journal:  Dev Cell       Date:  2015-09-10       Impact factor: 12.270

5.  Asymmetric properties of the Chlamydomonas reinhardtii cytoskeleton direct rhodopsin photoreceptor localization.

Authors:  Telsa M Mittelmeier; Joseph S Boyd; Mary Rose Lamb; Carol L Dieckmann
Journal:  J Cell Biol       Date:  2011-05-09       Impact factor: 10.539

6.  Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii.

Authors:  Susan K Dutcher; Linya Li; Huawen Lin; Leslie Meyer; Thomas H Giddings; Alan L Kwan; Brian L Lewis
Journal:  G3 (Bethesda)       Date:  2012-01-01       Impact factor: 3.154

7.  Structural organization of the intermediate and light chain complex of Chlamydomonas ciliary I1 dynein.

Authors:  Gang Fu; Chasity Scarbrough; Kangkang Song; Nhan Phan; Maureen Wirschell; Daniela Nicastro
Journal:  FASEB J       Date:  2021-06       Impact factor: 5.834

8.  The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length.

Authors:  Emily L Hunter; Karl Lechtreck; Gang Fu; Juyeon Hwang; Huawen Lin; Avanti Gokhale; Lea M Alford; Brian Lewis; Ryosuke Yamamoto; Ritsu Kamiya; Fan Yang; Daniela Nicastro; Susan K Dutcher; Maureen Wirschell; Winfield S Sale
Journal:  Mol Biol Cell       Date:  2018-03-29       Impact factor: 4.138

9.  Proteases Shape the Chlamydomonas Secretome: Comparison to Classical Neuropeptide Processing Machinery.

Authors:  Raj Luxmi; Crysten Blaby-Haas; Dhivya Kumar; Navin Rauniyar; Stephen M King; Richard E Mains; Betty A Eipper
Journal:  Proteomes       Date:  2018-09-23

10.  Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus.

Authors:  Gang Fu; Lei Zhao; Erin Dymek; Yuqing Hou; Kangkang Song; Nhan Phan; Zhiguo Shang; Elizabeth F Smith; George B Witman; Daniela Nicastro
Journal:  J Cell Biol       Date:  2019-10-31       Impact factor: 10.539
  10 in total

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