| Literature DB >> 20401760 |
Zhongdong Li1, Jie Zhao, Quanmin Li, Wenqi Yang, Qinglin Song, Wenyong Li, Junwen Liu.
Abstract
K562 cells and peripheral blood mononuclear cells were treated with hydrogen peroxide (H(2)O(2)) to determine the expression of Krüppel-like factor (KLF) 4. A full-length complementary DNA or an anti-sense oligonucleotide of KLF4 was transfected into cells, and expressions of B-cell lymphoma/leukemia-2 (bcl-2) and bcl-2-associated X (bax) proteins were analyzed. The results showed that H(2)O(2) treatment of cells resulted in an increase in KLF4 levels; KLF4 induced apoptosis and slowed cell growth, potentially resulting from up-regulation of bax and down-regulation of bcl-2. Transcriptional activities on bcl-2 and bax were promoted following KLF4 overexpression potentially through KLF4 binding sites on corresponding promoters. All results indicate that KLF4 induces apoptosis in leukemia cells involving the bcl-2/bax pathway during H(2)O(2) stimulation, suggesting a potential mechanism for research on drug-induced apoptosis.Entities:
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Year: 2010 PMID： 20401760 PMCID： PMC3024064 DOI： 10.1007/s12192-010-0199-5
Source DB: PubMed Journal: Cell Stress Chaperones ISSN： 1355-8145 Impact factor: 3.667