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Literature DB >> 2039450

The unfolding and refolding of pig heart fumarase.

Abstract

The unfolding of pig heart fumarase in solutions of guanidinium chloride (GdnHCl) has been examined. Loss of activity occurs at lower concentrations of GdnHCl than the structural changes detected by fluorescence or c.d. After denaturation, regain of activity can be observed provided that a reducing agent (dithiothreitol) is present and that the concentration of GdnHCl is lowered by dialysis rather than by dilution. The regain of secondary structure occurs with high efficiency even when little or no activity is recovered.

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Year: 1991 PMID： 2039450 PMCID： PMC1150116 DOI： 10.1042/bj2750745

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

23 in total

1. Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria.

Authors: M Y Cheng; F U Hartl; J Martin; R A Pollock; F Kalousek; W Neupert; E M Hallberg; R L Hallberg; A L Horwich
Journal: Nature Date: 1989-02-16 Impact factor: 49.962

2. Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis.

Authors: J Ostermann; A L Horwich; W Neupert; F U Hartl
Journal: Nature Date: 1989-09-14 Impact factor: 49.962

Review 3. Molecular chaperones: proteins essential for the biogenesis of some macromolecular structures.

Authors: R J Ellis; S M Hemmingsen
Journal: Trends Biochem Sci Date: 1989-08 Impact factor: 13.807

4. The preparation of guanidine hydrochloride.

Authors: Y Nozaki
Journal: Methods Enzymol Date: 1972 Impact factor: 1.600

5. Proline isomerization studied with proteolytic enzymes.

Authors: J F Brandts; L N Lin
Journal: Methods Enzymol Date: 1986 Impact factor: 1.600

Review 6. Folding and association of proteins.

Authors: R Jaenicke
Journal: Prog Biophys Mol Biol Date: 1987 Impact factor: 3.667

7. Estimation of globular protein secondary structure from circular dichroism.

Authors: S W Provencher; J Glöckner
Journal: Biochemistry Date: 1981-01-06 Impact factor: 3.162

8. Reconstitution of lactic dehydrogenase after acid dissociation. The yield of reactivation is determined by conformational rearrangements of the dissociated monomers.

Authors: G Zettlmeissl; R Rudolph; R Jaenicke
Journal: Eur J Biochem Date: 1981-12

9. Quasi-irreversibility in the unfolding-refolding transition of phosphoglycerate kinase induced by guanidine hydrochloride.

Authors: A Mitraki; J M Betton; M Desmadril; J M Yon
Journal: Eur J Biochem Date: 1987-02-16

10. The unfolding and refolding of glutamate dehydrogenases from bovine liver, baker's yeast and Clostridium symbosium.

Authors: S M West; N C Price
Journal: Biochem J Date: 1988-04-01 Impact factor: 3.857

9 in total

1. Enzyme activity after resealing within ghost erythrocyte cells, and protection by alpha-crystallin against fructose-induced inactivation.

Authors: Barry K Derham; John J Harding
Journal: Biochem J Date: 2002-12-15 Impact factor: 3.857

2. Dissociation and unfolding of Pi-class glutathione transferase. Evidence for a monomeric inactive intermediate.

Authors: A Aceto; A M Caccuri; P Sacchetta; T Bucciarelli; B Dragani; N Rosato; G Federici; C Di Ilio
Journal: Biochem J Date: 1992-07-01 Impact factor: 3.857

3. Dissociation and unfolding of the pyruvate dehydrogenase complex by guanidinium chloride.

Authors: S M West; J E Rice; E S Beaumont; S M Kelly; N C Price; J G Lindsay
Journal: Biochem J Date: 1995-06-15 Impact factor: 3.857

4. Inactivation and conformational changes of aminoacyclase in trifluoroethanol solutions.

Authors: Y X Zhang; S L Yan; H M Zhou
Journal: J Protein Chem Date: 1996-10

5. Comparison of inactivation and unfolding of green crab (Scylla serrata) alkaline phosphatase during denaturation by guanidinium chloride.

Authors: Q X Chen; W Zhang; W Z Zheng; Z Zhang; S X Yan; T Zhang; H M Zhou
Journal: J Protein Chem Date: 1996-05