PURPOSE: After the influence of different actions on the blood, the erythrocytes may change their macrostructure. At the same time, the microstructure of cell membrane will be changed as well. This study provides the results of comparison of red blood cell membrane microstructure after they have been affected by different factors. MATERIALS AND METHODS: Images and spatial profiles of the cell surface were obtained by atomic force microscope. It was proposed to use spatial Fourier transform to decompose the initial complex profile into series of simple ones. This made it possible to compare surface parameters after exposure of red blood cells to different external actions. RESULTS: Quantitative differences between membrane profile harmonic composition parameters (amplitude and spatial period) after physical impact (impulse electrical field, osmotic swelling) and after chemical impact (the fixing fluid glutaraldehyde and the drug Esmeron) were experimentally confirmed. CONCLUSIONS: Such experimental and theoretical approach may lay down the foundations of mechanisms of different factors' effect on red blood cells both in research and in clinics.
PURPOSE: After the influence of different actions on the blood, the erythrocytes may change their macrostructure. At the same time, the microstructure of cell membrane will be changed as well. This study provides the results of comparison of red blood cell membrane microstructure after they have been affected by different factors. MATERIALS AND METHODS: Images and spatial profiles of the cell surface were obtained by atomic force microscope. It was proposed to use spatial Fourier transform to decompose the initial complex profile into series of simple ones. This made it possible to compare surface parameters after exposure of red blood cells to different external actions. RESULTS: Quantitative differences between membrane profile harmonic composition parameters (amplitude and spatial period) after physical impact (impulse electrical field, osmotic swelling) and after chemical impact (the fixing fluid glutaraldehyde and the drug Esmeron) were experimentally confirmed. CONCLUSIONS: Such experimental and theoretical approach may lay down the foundations of mechanisms of different factors' effect on red blood cells both in research and in clinics.
Authors: Elena Kozlova; Alexander Chernysh; Victor Moroz; Olga Gudkova; Victoria Sergunova; Artem Kuzovlev Journal: Sci Rep Date: 2014-08-12 Impact factor: 4.379
Authors: Elena Kozlova; Viktoria Sergunova; Ekaterina Sherstyukova; Olga Gudkova; Aleksandr Kozlov; Vladimir Inozemtsev; Snezhanna Lyapunova; Aleksandr Chernysh Journal: Int J Mol Sci Date: 2022-02-12 Impact factor: 5.923
Authors: Andrea M López-Canizales; Aracely Angulo-Molina; Adriana Garibay-Escobar; Erika Silva-Campa; Miguel A Mendez-Rojas; Karla Santacruz-Gómez; Mónica Acosta-Elías; Beatriz Castañeda-Medina; Diego Soto-Puebla; Osiris Álvarez-Bajo; Alexel Burgara-Estrella; Martín Pedroza-Montero Journal: Front Physiol Date: 2021-06-04 Impact factor: 4.566