Literature DB >> 20377206

A consensus mechanism for Radical SAM-dependent dehydrogenation? BtrN contains two [4Fe-4S] clusters.

Tyler L Grove1, Jessica H Ahlum, Priya Sharma, Carsten Krebs, Squire J Booker.   

Abstract

BtrN catalyzes the two-electron oxidation of the C3 secondary alcohol of 2-deoxy-scyllo-inosamine to the corresponding ketone and is a member of a subclass of radical S-adenosylmethionine (SAM) enzymes called radical SAM (RS) dehydrogenases. Like all RS enzymes, BtrN contains a [4Fe-4S] cluster that delivers an electron to SAM, inducing its cleavage to the common intermediate in RS reactions, the 5'-deoxyadenosyl 5'-radical. In this work, we show that BtrN contains an additional [4Fe-4S] cluster, thought to bind in contact with the substrate to facilitate loss of the second electron in the two-electron oxidation.

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Year:  2010        PMID： 20377206      PMCID： PMC3598582          DOI： 10.1021/bi9022126

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  12 in total

1.  Anaerobic sulfatase-maturating enzymes: radical SAM enzymes able to catalyze in vitro sulfatase post-translational modification.

Authors:  Alhosna Benjdia; Jérôme Leprince; Alain Guillot; Hubert Vaudry; Sylvie Rabot; Olivier Berteau
Journal:  J Am Chem Soc       Date:  2007-03-03       Impact factor: 15.419

2.  Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy.

Authors:  Kenichi Yokoyama; Daijiro Ohmori; Fumitaka Kudo; Tadashi Eguchi
Journal:  Biochemistry       Date:  2008-08-02       Impact factor: 3.162

3.  The iron sulfur protein AtsB is required for posttranslational formation of formylglycine in the Klebsiella sulfatase.

Authors:  C Szameit; C Miech; M Balleininger; B Schmidt; K von Figura; T Dierks
Journal:  J Biol Chem       Date:  1999-05-28       Impact factor: 5.157

4.  Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods.

Authors:  H J Sofia; G Chen; B G Hetzler; J F Reyes-Spindola; N E Miller
Journal:  Nucleic Acids Res       Date:  2001-03-01       Impact factor: 16.971

Review 5.  Anaerobic functionalization of unactivated C-H bonds.

Authors:  Squire J Booker
Journal:  Curr Opin Chem Biol       Date:  2009-03-16       Impact factor: 8.822

6.  Coordination and mechanism of reversible cleavage of S-adenosylmethionine by the [4Fe-4S] center in lysine 2,3-aminomutase.

Authors:  Dawei Chen; Charles Walsby; Brian M Hoffman; Perry A Frey
Journal:  J Am Chem Soc       Date:  2003-10-01       Impact factor: 15.419

7.  Characterization and mechanistic study of a radical SAM dehydrogenase in the biosynthesis of butirosin.

Authors:  Kenichi Yokoyama; Mario Numakura; Fumitaka Kudo; Daijiro Ohmori; Tadashi Eguchi
Journal:  J Am Chem Soc       Date:  2007-11-15       Impact factor: 15.419

8.  Post-translational formylglycine modification of bacterial sulfatases by the radical S-adenosylmethionine protein AtsB.

Authors:  Qinghua Fang; Jianhe Peng; Thomas Dierks
Journal:  J Biol Chem       Date:  2004-01-28       Impact factor: 5.157

9.  In vitro characterization of AtsB, a radical SAM formylglycine-generating enzyme that contains three [4Fe-4S] clusters.

Authors:  Tyler L Grove; Kyung-Hoon Lee; Jennifer St Clair; Carsten Krebs; Squire J Booker
Journal:  Biochemistry       Date:  2008-06-18       Impact factor: 3.162

10.  A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency.

Authors:  B Schmidt; T Selmer; A Ingendoh; K von Figura
Journal:  Cell       Date:  1995-07-28       Impact factor: 41.582
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  39 in total

1.  Mechanistic studies of the radical S-adenosyl-L-methionine enzyme DesII: EPR characterization of a radical intermediate generated during its catalyzed dehydrogenation of TDP-D-quinovose.

Authors:  Mark W Ruszczycky; Sei-hyun Choi; Steven O Mansoorabadi; Hung-wen Liu
Journal:  J Am Chem Soc       Date:  2011-04-22       Impact factor: 15.419

2.  The radical SAM enzyme AlbA catalyzes thioether bond formation in subtilosin A.

Authors:  Leif Flühe; Thomas A Knappe; Michael J Gattner; Antje Schäfer; Olaf Burghaus; Uwe Linne; Mohamed A Marahiel
Journal:  Nat Chem Biol       Date:  2012-02-26       Impact factor: 15.040

3.  Spectroscopic and Electrochemical Characterization of the Mycofactocin Biosynthetic Protein, MftC, Provides Insight into Its Redox Flipping Mechanism.

Authors:  Richard Ayikpoe; Thacien Ngendahimana; Michelle Langton; Sheila Bonitatibus; Lindsey M Walker; Sandra S Eaton; Gareth R Eaton; Maria-Eirini Pandelia; Sean J Elliott; John A Latham
Journal:  Biochemistry       Date:  2019-01-25       Impact factor: 3.162

4.  Mechanistic Enzymology of the Radical SAM Enzyme DesII.

Authors:  Mark W Ruszczycky; Hung-Wen Liu
Journal:  Isr J Chem       Date:  2015-02-20       Impact factor: 3.333

5.  X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification.

Authors:  Peter J Goldman; Tyler L Grove; Lauren A Sites; Martin I McLaughlin; Squire J Booker; Catherine L Drennan
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-06       Impact factor: 11.205

6.  Probing the reaction mechanism of spore photoproduct lyase (SPL) via diastereoselectively labeled dinucleotide SP TpT substrates.

Authors:  Linlin Yang; Gengjie Lin; Degang Liu; Karl J Dria; Joshua Telser; Lei Li
Journal:  J Am Chem Soc       Date:  2011-06-14       Impact factor: 15.419

Review 7.  Biogenesis of the peptide-derived redox cofactor pyrroloquinoline quinone.

Authors:  Wen Zhu; Judith P Klinman
Journal:  Curr Opin Chem Biol       Date:  2020-07-27       Impact factor: 8.822

8.  EPR-kinetic isotope effect study of the mechanism of radical-mediated dehydrogenation of an alcohol by the radical SAM enzyme DesII.

Authors:  Mark W Ruszczycky; Sei-hyun Choi; Hung-wen Liu
Journal:  Proc Natl Acad Sci U S A       Date:  2013-01-17       Impact factor: 11.205

9.  X-ray analysis of butirosin biosynthetic enzyme BtrN redefines structural motifs for AdoMet radical chemistry.

Authors:  Peter J Goldman; Tyler L Grove; Squire J Booker; Catherine L Drennan
Journal:  Proc Natl Acad Sci U S A       Date:  2013-09-18       Impact factor: 11.205

10.  Mechanistic studies of the spore photoproduct lyase via a single cysteine mutation.

Authors:  Linlin Yang; Gengjie Lin; Renae S Nelson; Yajun Jian; Joshua Telser; Lei Li
Journal:  Biochemistry       Date:  2012-08-31       Impact factor: 3.162
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