Literature DB >> 20351207

Development and evaluation of loop-mediated isothermal amplification assay for rapid and inexpensive detection of cytomegalovirus DNA in vitreous specimens from suspected cases of viral retinitis.

Ashok Kumar Reddy1, Praveen Kumar Balne, Rajeev Kumar Reddy, Annie Mathai, Inderjeet Kaur.   

Abstract

A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of cytomegalovirus (CMV) was developed and evaluated. The LAMP assay specifically amplified only CMV DNA, and no cross-reactivity with the DNA of herpes simplex virus type 1, varicella-zoster virus, adenovirus, Aspergillus flavus, or Staphylococcus aureus was observed. The sequences of the LAMP assay-positive CMV products were perfectly (100%) matched with the CMV sequence deposited in the GenBank database. The sensitivity of the LAMP assay was found to be 10 copies/microl of CMV DNA. Vitreous samples from 40 patients with suspected retinitis were subjected to LAMP and real-time PCR for the detection of CMV. Of 40 patients with suspected viral retinitis, 10 tested positive for CMV by the real-time PCR and LAMP assays. A 100% concordance was observed between the results of the two methods. The LAMP assay is a rapid, highly specific, and sensitive method for the diagnosis of retinitis caused by CMV.

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Year:  2010        PMID： 20351207      PMCID： PMC2884485          DOI： 10.1128/JCM.02248-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Loop-mediated isothermal amplification of DNA.

Authors:  T Notomi; H Okayama; H Masubuchi; T Yonekawa; K Watanabe; N Amino; T Hase
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

2.  Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus.

Authors:  Manmohan Parida; Guillermo Posadas; Shingo Inoue; Futoshi Hasebe; Kouichi Morita
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948

3.  Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay.

Authors:  Manmohan Parida; Kouhei Horioke; Hiroyuki Ishida; Paban Kumar Dash; Parag Saxena; Asha Mukul Jana; Mohammed Alimul Islam; Shingo Inoue; Norimitsu Hosaka; Kouichi Morita
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

4.  Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection.

Authors:  Leo L M Poon; Bonnie W Y Wong; Kwok H Chan; Stella S F Ng; Kwok Y Yuen; Yi Guan; J S Malik Peiris
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

5.  Detection of herpesvirus DNA in vitreous and aqueous specimens by the polymerase chain reaction.

Authors:  G M Fox; C A Crouse; E L Chuang; S C Pflugfelder; T J Cleary; S J Nelson; S S Atherton
Journal:  Arch Ophthalmol       Date:  1991-02

Review 6.  Current perspectives of herpesviral retinitis and choroiditis.

Authors:  H N Madhavan; K Priya; J Biswas
Journal:  Indian J Pathol Microbiol       Date:  2004-10       Impact factor: 0.740

7.  Acquired immunodeficiency syndrome--associated herpes simplex virus retinitis. Clinical description and use of a polymerase chain reaction--based assay as a diagnostic tool.

Authors:  E T Cunningham; G A Short; A R Irvine; J S Duker; T P Margolis
Journal:  Arch Ophthalmol       Date:  1996-07

8.  Serologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis.

Authors:  J H de Boer; C Verhagen; M Bruinenberg; A Rothova; P T de Jong; G S Baarsma; A Van der Lelij; F M Ooyman; J G Bollemeijer; P J Derhaag; A Kijlstra
Journal:  Am J Ophthalmol       Date:  1996-06       Impact factor: 5.258

9.  A sensitive and specific polymerase chain reaction-based assay for the diagnosis of cytomegalovirus retinitis.

Authors:  J D McCann; T P Margolis; M G Wong; B D Kuppermann; A P Luckie; D M Schwartz; A R Irvine; E Ai
Journal:  Am J Ophthalmol       Date:  1995-08       Impact factor: 5.258

Review 10.  Herpetic retinitis.

Authors:  Miguel Cordero-Coma; Fahd Anzaar; Taygan Yilmaz; C Stephen Foster
Journal:  Herpes       Date:  2007-06
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  4 in total

1.  Development of a loop-mediated isothermal amplification assay targeting the mpb64 gene for diagnosis of intraocular tuberculosis.

Authors:  Praveen Kumar Balne; Manas Ranjan Barik; Savitri Sharma; Soumyava Basu
Journal:  J Clin Microbiol       Date:  2013-08-21       Impact factor: 5.948

2.  Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71.

Authors:  Weifeng Shi; Kun Li; Yun Ji; Qingbo Jiang; Mei Shi; Zuhuang Mi
Journal:  BMC Infect Dis       Date:  2011-07-18       Impact factor: 3.090

Review 3.  Global Dissemination of Carbapenemase-Producing Klebsiella pneumoniae: Epidemiology, Genetic Context, Treatment Options, and Detection Methods.

Authors:  Chang-Ro Lee; Jung Hun Lee; Kwang Seung Park; Young Bae Kim; Byeong Chul Jeong; Sang Hee Lee
Journal:  Front Microbiol       Date:  2016-06-13       Impact factor: 5.640

4.  Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection.

Authors:  Yago Gomes; Adele Caterino-de-Araujo; Karoline Campos; Maria Gisele Gonçalves; Ana Claudia Leite; Marco Antonio Lima; Abelardo Araújo; Marcus Tulius Silva; Otávio Espíndola
Journal:  Viruses       Date:  2020-09-04       Impact factor: 5.048
  4 in total

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