Localization of CART-positive neurons in the amygdaloid body and the relationship between their immunoreactivity and the sex steroid level.

This is the first study reporting the locations of neurons expressing CART peptide (cocaine-amphetamine-regulated transcript) throughout all the nuclear and paleocortical formations of the amygdaloid body (AB) and demonstrating the effects of sex steroids on immunoreactivity. The immunocytochemical reaction was performed on frontal brain sections from adult rats (seven females in estrus, seven females in metestrus, and seven males). The proportions of immunoreactive neurons to the total number of neurons in adjacent sections stained by the Nissl method were assessed in estrus and metestrus. In the dorsomedial and posterior cortical nuclei and the lateral capsular subnucleus of the central field, the relative numbers of immunoreactive neurons at estrus were significantly greater than at metestrus. These results provide evidence of the involvement of the olfactory and integrative centers of the AB in the pathogenesis of drug dependence and show that new and efficient methods of gene therapy might be developed using the intranasal route for drug administration.