Literature DB >> 20336673

Improved ethanol precipitation of DNA.

Rosa Fregel1, Ana González, Vicente M Cabrera.   

Abstract

In this Short Communication, a shorter version of the standard DNA ethanol precipitation and purification protocol is described. It uses a mixture of 70% ethanol, 75 mM ammonium acetate and different concentrations of different carriers to perform DNA precipitation and washing in only one step.

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Year:  2010        PMID： 20336673     DOI： 10.1002/elps.200900721

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  7 in total

1.  Evaluation and statistical optimization of a method for methylated cell-free fetal DNA extraction from maternal plasma.

Authors:  Mostafa Akbariqomi; Reza Heidari; Soraya Saleh Gargari; Mir Davood Omrani; Garshasb Rigi; Nafiseh Sadat Sanikhani; Hamid Kooshki; Fatemeh Mahmoudian; Mohammad Ali Mazlomi; Gholamreza Tavoosidana
Journal:  J Assist Reprod Genet       Date:  2019-02-28       Impact factor: 3.412

2.  A modified approach for high-quality RNA extraction of spore-forming Bacillus subtilis at varied physiological stages.

Authors:  Phetcharat Jaiaue; Piroonporn Srimongkol; Sitanan Thitiprasert; Somboon Tanasupawat; Benjamas Cheirsilp; Suttichai Assabumrungrat; Nuttha Thongchul
Journal:  Mol Biol Rep       Date:  2021-08-28       Impact factor: 2.316

3.  Mineralization content alters osteogenic responses of bone marrow stromal cells on hydroxyapatite/polycaprolactone composite nanofiber scaffolds.

Authors:  Timothy T Ruckh; Derek A Carroll; Justin R Weaver; Ketul C Popat
Journal:  J Funct Biomater       Date:  2012-11-14

4.  Highly Concentrated Ethanol Solutions: Good Solvents for DNA as Revealed by Single-Molecule Observation.

Authors:  Yuki Oda; Koichiro Sadakane; Yuko Yoshikawa; Tadayuki Imanaka; Kingo Takiguchi; Masahito Hayashi; Takahiro Kenmotsu; Kenichi Yoshikawa
Journal:  Chemphyschem       Date:  2016-01-13       Impact factor: 3.102

5.  Mutational analysis of CYP1B1 (rs56010818) variant in primary open angle glaucoma (POAG) affected patients of Pakistan.

Authors:  Ashok Kumar Narsani; Ali Muhammad Waryah; Muhammad Rafiq; Hina Shaikh; Syed Habib Ahmed Naqvi; Raveet Kumar; Pawan Kumar
Journal:  Saudi J Biol Sci       Date:  2021-08-25       Impact factor: 4.219

6.  Development of an Automatable Affinity Purification Process for DNA-Encoded Chemistry.

Authors:  Katharina Götte; Robin Dinter; Leon Justen; Norbert Kockmann; Andreas Brunschweiger
Journal:  ACS Omega       Date:  2022-08-02

7.  Development of ssDNA Aptamers for Diagnosis and Inhibition of the Highly Pathogenic Avian Influenza Virus Subtype H5N1.

Authors:  Sang-Heon Kim; Jae-Woo Choi; A-Ru Kim; Sang-Choon Lee; Moon-Young Yoon
Journal:  Biomolecules       Date:  2020-07-28
  7 in total

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