Yiyan Liu1, Lionel S Zuckier, Nasrin V Ghesani. 1. Section of Nuclear Medicine, Department of Radiology, 150 Bergen Street, H-141, New Jersey Medical School, UMDNJ, Newark, New Jersey 07103, USA. liuyl@umdnj.edu
Abstract
BACKGROUND: Prostate cancer is characterized by a low rate of glycolysis and glucose uptake. We hypothesize that fatty acid is dominant over glucose in uptake by prostate cells. MATERIALS AND METHODS: One benign (RWPE1) and two malignant (LNCaP and PC3) prostate cell lines were assayed for their in vitro uptake of radiolabeled glucose analogs (3)H-fluoro-2-deoxyglucose and (18)F-fluoro-2-deoxyglucose, and the long-chain fatty acid (3)H-palmitic acid. Fractional uptake was standardized to viable cell numbers. RESULTS: Uptake of palmitate in all 3 prostate cell lines was significantly higher than that of glucose at all incubation times (p<0.01). But in malignant cell lines, neither glucose nor palmitate uptake was quantitatively higher than that in the benign cell line. The uptake of fatty acid by prostate cells is a dynamic, active process mediated by the membrane receptors. CONCLUSION: Prostate cells are characterized by a dominant uptake of fatty acid over glucose, suggesting that future development of new diagnostic and therapeutic approaches in prostatic cancer should focus on fatty acid substrate. Fatty acid imaging may be useful in detection of recurrence and metastasis, but not in differentiating malignant from benign prostate tissue.
BACKGROUND:Prostate cancer is characterized by a low rate of glycolysis and glucose uptake. We hypothesize that fatty acid is dominant over glucose in uptake by prostate cells. MATERIALS AND METHODS: One benign (RWPE1) and two malignant (LNCaP and PC3) prostate cell lines were assayed for their in vitro uptake of radiolabeled glucose analogs (3)H-fluoro-2-deoxyglucose and (18)F-fluoro-2-deoxyglucose, and the long-chain fatty acid (3)H-palmitic acid. Fractional uptake was standardized to viable cell numbers. RESULTS: Uptake of palmitate in all 3 prostate cell lines was significantly higher than that of glucose at all incubation times (p<0.01). But in malignant cell lines, neither glucose nor palmitate uptake was quantitatively higher than that in the benign cell line. The uptake of fatty acid by prostate cells is a dynamic, active process mediated by the membrane receptors. CONCLUSION: Prostate cells are characterized by a dominant uptake of fatty acid over glucose, suggesting that future development of new diagnostic and therapeutic approaches in prostatic cancer should focus on fatty acid substrate. Fatty acid imaging may be useful in detection of recurrence and metastasis, but not in differentiating malignant from benign prostate tissue.
Authors: Natalie J German; Haejin Yoon; Rushdia Z Yusuf; J Patrick Murphy; Lydia W S Finley; Gaëlle Laurent; Wilhelm Haas; F Kyle Satterstrom; Jlenia Guarnerio; Elma Zaganjor; Daniel Santos; Pier Paolo Pandolfi; Andrew H Beck; Steven P Gygi; David T Scadden; William G Kaelin; Marcia C Haigis Journal: Mol Cell Date: 2016-09-15 Impact factor: 17.970
Authors: Yan Shi; Jenny J Han; Jayantha B Tennakoon; Fabiola F Mehta; Fatima A Merchant; Alan R Burns; Matthew K Howe; Donald P McDonnell; Daniel E Frigo Journal: Mol Endocrinol Date: 2012-12-18
Authors: Amin Khalifeh-Soltani; William McKleroy; Stephen Sakuma; Yuk Yin Cheung; Kevin Tharp; Yifu Qiu; Scott M Turner; Ajay Chawla; Andreas Stahl; Kamran Atabai Journal: Nat Med Date: 2014-01-19 Impact factor: 53.440
Authors: Soumen K Manna; Naoki Tanaka; Kristopher W Krausz; Majda Haznadar; Xiang Xue; Tsutomu Matsubara; Elise D Bowman; Eric R Fearon; Curtis C Harris; Yatrik M Shah; Frank J Gonzalez Journal: Gastroenterology Date: 2014-01-15 Impact factor: 22.682