Literature DB >> 2030964

Overexpression, purification and crystallization of BamHI endonuclease.

W E Jack1, L Greenough, L F Dorner, S Y Xu, T Strzelecka, A K Aggarwal, I Schildkraut.   

Abstract

The type II restriction endonuclease BamHI has been expressed in E. coli, producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which diffracts to at least 1.9 A in X-ray analysis.

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Year:  1991        PMID： 2030964      PMCID： PMC328111          DOI： 10.1093/nar/19.8.1825

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  20 in total

1.  The time-resolved kinetics of superhelical DNA cleavage by BamHI restriction endonuclease.

Authors:  P Hensley; G Nardone; J G Chirikjian; M E Wastney
Journal:  J Biol Chem       Date:  1990-09-05       Impact factor: 5.157

2.  Refinement of Eco RI endonuclease crystal structure: a revised protein chain tracing.

Authors:  Y C Kim; J C Grable; R Love; P J Greene; J M Rosenberg
Journal:  Science       Date:  1990-09-14       Impact factor: 47.728

3.  Altering the specificity of restriction endonuclease: effect of replacing Mg2+ with Mn2+.

Authors:  M Hsu; P Berg
Journal:  Biochemistry       Date:  1978-01-10       Impact factor: 3.162

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

6.  Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

Authors:  W B Wood
Journal:  J Mol Biol       Date:  1966-03       Impact factor: 5.469

7.  Recognition sequence of specific endonuclease BamH.I from Bacillus amyloliquefaciens H.

Authors:  R J Roberts; G A Wilson; F E Young
Journal:  Nature       Date:  1977-01-06       Impact factor: 49.962

8.  A rapid boiling method for the preparation of bacterial plasmids.

Authors:  D S Holmes; M Quigley
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

9.  Sequence-specific endonuclease Bam HI. Effect of hydrophobic reagents on sequence recognition and catalysis.

Authors:  J George; R W Blakesley; J G Chirikjian
Journal:  J Biol Chem       Date:  1980-07-25       Impact factor: 5.157

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  8 in total

1.  The regulatory C proteins from different restriction-modification systems can cross-complement.

Authors:  C L Ives; A Sohail; J E Brooks
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

2.  Direct selection of binding proficient/catalytic deficient variants of BamHI endonuclease.

Authors:  L F Dorner; I Schildkraut
Journal:  Nucleic Acids Res       Date:  1994-03-25       Impact factor: 16.971

3.  Three-dimensional structure of the adenine-specific DNA methyltransferase M.Taq I in complex with the cofactor S-adenosylmethionine.

Authors:  J Labahn; J Granzin; G Schluckebier; D P Robinson; W E Jack; I Schildkraut; W Saenger
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-08       Impact factor: 11.205

4.  Regulation of the BamHI restriction-modification system by a small intergenic open reading frame, bamHIC, in both Escherichia coli and Bacillus subtilis.

Authors:  C L Ives; P D Nathan; J E Brooks
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

5.  Cofactor requirements of BamHI mutant endonuclease E77K and its suppressor mutants.

Authors:  S Y Xu; I Schildkraut
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

6.  Restriction Cascade Exponential Amplification (RCEA) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qPCR.

Authors:  Andrey L Ghindilis; Maria W Smith; Holly M Simon; Ihab A Seoudi; Nina S Yazvenko; Iain A Murray; Xiaoqing Fu; Kenneth Smith; Linda Jen-Jacobson; Shuang-Yong Xu
Journal:  Sci Rep       Date:  2015-01-13       Impact factor: 4.379

7.  Engineering Infrequent DNA Nicking Endonuclease by Fusion of a BamHI Cleavage-Deficient Mutant and a DNA Nicking Domain.

Authors:  Shuang-Yong Xu
Journal:  Front Microbiol       Date:  2022-02-01       Impact factor: 5.640

8.  Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI.

Authors:  Shuang-Yong Xu; Zhenyu Zhu; Penghua Zhang; Siu-Hong Chan; James C Samuelson; Jianping Xiao; Debra Ingalls; Geoffrey G Wilson
Journal:  Nucleic Acids Res       Date:  2007-06-22       Impact factor: 16.971
  8 in total

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