Literature DB >> 2029338

Construction and characterization of a partial library of yeast artificial chromosomes from human chromosome 21.

M Bellis1, A Gérard, J P Charlieu, B Marçais, M E Brun, E Viegas-Péquignot, D A Carter, G Roizès.   

Abstract

We report a protocol for cloning large DNA fragments in yeast artificial chromosomes (YAC). A partial library has been constructed from a somatic hybrid containing chromosome 21 as the single source of human DNA. About 4.0 Mb of human DNA was recovered in 17 YAC clones. Three clones were analyzed by in situ hybridization and mapped on chromosome 21. One clone hybridized with the chromosome 21 centromeric region and may provide new insight both on the molecular structure of centromere and on the localization of Alzheimer disease gene.

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Year:  1991        PMID： 2029338     DOI： 10.1089/dna.1991.10.301

Source DB:  PubMed          Journal:  DNA Cell Biol        ISSN： 1044-5498            Impact factor:   3.311


  5 in total

1.  Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

Authors:  H Shizuya; B Birren; U J Kim; V Mancino; T Slepak; Y Tachiiri; M Simon
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

2.  3' Alu PCR: a simple and rapid method to isolate human polymorphic markers.

Authors:  J P Charlieu; A M Laurent; D A Carter; M Bellis; G Roizès
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

3.  A human chromosome 7-specific genomic DNA library in yeast artificial chromosomes.

Authors:  S W Scherer; B J Tompkins; L C Tsui
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

4.  Analysis of pericentromeric chromosome 21 specific YAC clones by FISH: identification of new markers for molecular-cytogenetic application.

Authors:  Y B Yurov; A M Laurent; B Marcais; S G Vorsanova; G Roizes
Journal:  Hum Genet       Date:  1995-03       Impact factor: 4.132

Review 5.  Yeast artificial chromosome cloning.

Authors:  M Ramsay
Journal:  Mol Biotechnol       Date:  1994-04       Impact factor: 2.695
  5 in total

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