Literature DB >> 20246

A kinetic spectrophotometric assay for rapid determination of acetoacetate in blood.

C P Price, B Llyod, G M Alberti.   

Abstract

We describe an automated kinetic assay for acetoacetate in blood. Acetoacetate is enzymatically reduced to D-beta-hydroxybutyrate and the reaction is monitored for 60 s with a reaction-rate analyzer. This technique allows low concentrations of acetoacetate to be measured with good precision and overcomes many of the problems associated with other automated techniques. Our studies on the stability of acetoacetate emphasize the need for care in handling specimens. The use of a reaction-rate analyzer, an item of equipment common to most laboratories, allows for rapid handling of samples in small or large batches, depending on the needs of the laboratory.

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Year:  1977        PMID： 20246

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  23 in total

1.  High blood ketone body concentration in type 2 non-insulin dependent diabetic patients.

Authors:  A Avogaro; C Crepaldi; M Miola; A Maran; V Pengo; A Tiengo; S Del Prato
Journal:  J Endocrinol Invest       Date:  1996-02       Impact factor: 4.256

2.  Metabolic and hormonal responses during squash.

Authors:  G Garden; P J Hale; P M Horrocks; J Crase; V Hammond; M Nattrass
Journal:  Eur J Appl Physiol Occup Physiol       Date:  1986

3.  Cardiovascular and metabolic responses to noradrenaline in men acclimatized to cold baths.

Authors:  G M Budd; J R Brotherhood; D W Thomas; F A Beasley; A L Hendrie; S E Jeffery; G J Lincoln; A T Solaga
Journal:  Eur J Appl Physiol Occup Physiol       Date:  1993

4.  Metabolic effects of bicarbonate in the treatment of diabetic ketoacidosis.

Authors:  P J Hale; J Crase; M Nattrass
Journal:  Br Med J (Clin Res Ed)       Date:  1984-10-20

5.  The metabolic effects of moderately severe upper gastrointestinal haemorrhage in man.

Authors:  K J Foster; K G Alberti; C Binder; G Holdstock; S J Karran; C L Smith; S Talbot; D C Turnell
Journal:  Postgrad Med J       Date:  1982-01       Impact factor: 2.401

6.  The effect of continuous subcutaneous insulin infusion and conventional insulin regimes on 24-hour variations of blood glucose and intermediary metabolites in the third trimester of diabetic pregnancy.

Authors:  J M Potter; J P Reckless; D R Cullen
Journal:  Diabetologia       Date:  1981-12       Impact factor: 10.122

7.  Metabolic effects of acute and prolonged growth hormone excess in normal and insulin-deficient man.

Authors:  P Metcalfe; D G Johnston; R Nosadini; H Orksov; K G Alberti
Journal:  Diabetologia       Date:  1981-02       Impact factor: 10.122

8.  Diurnal variations in blood intermediary metabolites in mild gestational diabetic patients and the effect of a carbohydrate-restricted diet.

Authors:  J M Potter; J P Reckless; D R Cullen
Journal:  Diabetologia       Date:  1982-02       Impact factor: 10.122

9.  Ketone bodies increase glomerular filtration rate in normal man and in patients with type 1 (insulin-dependent) diabetes mellitus.

Authors:  R Trevisan; R Nosadini; P Fioretto; A Avogaro; E Duner; E Jori; A Valerio; A Doria; G Crepaldi
Journal:  Diabetologia       Date:  1987-04       Impact factor: 10.122

10.  Glucose turnover and recycling in colorectal carcinoma.

Authors:  W A Kokal; A McCulloch; P D Wright; I D Johnston
Journal:  Ann Surg       Date:  1983-11       Impact factor: 12.969
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