Literature DB >> 20238277

Evaluation of antifungal susceptibility using flow cytometry.

Cidália Pina-Vaz1, Acácio Gonçalves Rodrigues.   

Abstract

Flow cytometry has found wide applications in areas like haematology and immunology, but also presents great potential in microbiology. The susceptibility of clinical isolates of Candida and Cryptococcus to antifungal compounds can be assayed by flow cytometry using fluorescent probes like FUN-1, propidium iodide and JC-1, with several advantages. Following 1 or 2 h of incubation, depending on the antifungal compound, versus 48 h of the classical methods, it is possible to establish the different susceptibility profiles. Additionally, it provides information regarding the mechanisms of action and might infer about resistance mechanisms.

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Year:  2010        PMID: 20238277     DOI: 10.1007/978-1-60761-611-5_21

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  7 in total

1.  Novel method for evaluating in vitro activity of anidulafungin in combination with amphotericin B or azoles.

Authors:  R Teixeira-Santos; R Rocha; A Moreira-Rosário; M Monteiro-Soares; E Cantón; A G Rodrigues; C Pina-Vaz
Journal:  J Clin Microbiol       Date:  2012-06-12       Impact factor: 5.948

2.  Synergistic effect of the flavonoid catechin, quercetin, or epigallocatechin gallate with fluconazole induces apoptosis in Candida tropicalis resistant to fluconazole.

Authors:  Cecília Rocha da Silva; João Batista de Andrade Neto; Rosana de Sousa Campos; Narjara Silvestre Figueiredo; Letícia Serpa Sampaio; Hemerson Iury Ferreira Magalhães; Bruno Coêlho Cavalcanti; Danielle Macêdo Gaspar; Geanne Matos de Andrade; Iri Sandro Pampolha Lima; Glauce Socorro de Barros Viana; Manoel Odorico de Moraes; Marina Duarte Pinto Lobo; Thalles Barbosa Grangeiro; Hélio Vitoriano Nobre Júnior
Journal:  Antimicrob Agents Chemother       Date:  2013-12-23       Impact factor: 5.191

3.  Synergistic effects of amiodarone and fluconazole on Candida tropicalis resistant to fluconazole.

Authors:  Cecília Rocha da Silva; João Batista de Andrade Neto; José Júlio Costa Sidrim; Maria Rozzelê Ferreira Angelo; Hemerson Iury Ferreira Magalhães; Bruno Coêlho Cavalcanti; Raimunda Sâmia Nogueira Brilhante; Danielle Silveira Macedo; Manoel Odorico de Moraes; Marina Duarte Pinto Lobo; Thalles Barbosa Grangeiro; Hélio Vitoriano Nobre Júnior
Journal:  Antimicrob Agents Chemother       Date:  2013-01-28       Impact factor: 5.191

4.  Synergistic antimicrobial action of chlorhexidine and ozone in endodontic treatment.

Authors:  Rita Noites; Cidália Pina-Vaz; Rita Rocha; Manuel Fontes Carvalho; Acácio Gonçalves; Irene Pina-Vaz
Journal:  Biomed Res Int       Date:  2014-05-28       Impact factor: 3.411

5.  A Flow Cytometric and Computational Approaches to Carbapenems Affinity to the Different Types of Carbapenemases.

Authors:  Cidália Pina-Vaz; Ana P Silva; Isabel Faria-Ramos; Rita Teixeira-Santos; Daniel Moura; Tatiana F Vieira; Sérgio F Sousa; Sofia Costa-de-Oliveira; Rafael Cantón; Acácio G Rodrigues
Journal:  Front Microbiol       Date:  2016-08-09       Impact factor: 5.640

6.  Potential Impact of Flow Cytometry Antimicrobial Susceptibility Testing on the Clinical Management of Gram-Negative Bacteremia Using the FASTinov® Kit.

Authors:  Sofia Costa-de-Oliveira; Rita Teixeira-Santos; Ana P Silva; Elika Pinho; Paulo Mergulhão; Ana Silva-Dias; Nádia Marques; Inês Martins-Oliveira; Acácio G Rodrigues; José A Paiva; Rafael Cantón; Cidália Pina-Vaz
Journal:  Front Microbiol       Date:  2017-12-12       Impact factor: 5.640

7.  The Vacuole and Mitochondria Patch (vCLAMP) Protein Mcp1 Is Involved in Maintenance of Mitochondrial Function and Mitophagy in Candida albicans.

Authors:  Xiaolong Mao; Li Yang; Yiming Fan; Jiazhen Wang; Dongkai Cui; Dixiong Yu; Qilin Yu; Mingchun Li
Journal:  Front Microbiol       Date:  2021-02-04       Impact factor: 5.640

  7 in total

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