Literature DB >> 202261

Rapid isolation of plasma membranes in high yield from cultured fibroblasts.

D Thom, A J Powell, C W Lloyd, D A Rees.   

Abstract

1. A method was developed which allows the rapid preparation of pure plasma membranes in high yield from cultured fibroblasts. 2. Cells are lysed in hypo-osmotic borate/EDTA and, after differential centrifugation, the membranes collected by centrifugation on a sucrose barrier. 3. Electron microscopy of the isolated material shows large membrane vesicles essentially free from contaminating organelles. 4. There is no detectable activity of the endoplasmic-reticulum enzyme marker, NADH2--lipoamide oxidoreductase (EC 1.6.4.3), and that of succinate dehydrogenase (EC 1.3.99.1), a marker for mitochondria, is substantially decreased. Chemical compositions are in good agreement with previous observations. 5. This study confirms the usefulness of applied isotopic markers for isolating plasma membranes.

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Year:  1977        PMID： 202261      PMCID： PMC1183751          DOI： 10.1042/bj1680187

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  27 in total

1.  ISOLATION OF A MUCOPEPTIDE FROM THE SURFACE OF EHRLICH ASCITES TUMOUR CELLS.

Authors:  O K LANGLEY; E J AMBROSE
Journal:  Nature       Date:  1964-10-03       Impact factor: 49.962

2.  A sialomucopeptide liberated by trypsin from the human erythrocyte.

Authors:  G M COOK; D H HEARD; G V SEAMAN
Journal:  Nature       Date:  1960-12-17       Impact factor: 49.962

3.  Studies on sialic acids.

Authors:  G BLIX; E LINDBERG; L ODIN; I WERNER
Journal:  Acta Soc Med Ups       Date:  1956-06-30

4.  A new method for the direct determination of serum cholesterol.

Authors:  A ZLATKIS; B ZAK; A J BOYLE
Journal:  J Lab Clin Med       Date:  1953-03

5.  Membrane receptors as general markers for plasma membrane isolation procedures. The use of 125-I-labeled wheat germ agglutinin, insulin, and cholera toxin.

Authors:  K J Chang; V Bennett; P Cuatrecasas
Journal:  J Biol Chem       Date:  1975-01-25       Impact factor: 5.157

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots.

Authors:  G Rouser; A N Siakotos; S Fleischer
Journal:  Lipids       Date:  1966-01       Impact factor: 1.880

8.  Cell surface and metabolic labelling of the proteins of normal and transformed chicken cells.

Authors:  D R Critchley; J A Wyke; R O Hynes
Journal:  Biochim Biophys Acta       Date:  1976-06-17

9.  The isolation and characterization of the plasma membrane of cultured chinese hamster ovary cells.

Authors:  R L Juliano; E Gagalang
Journal:  Can J Biochem       Date:  1975-08

10.  THE ISOLATION AND CHARACTERIZATION OF SUBCELLULAR COMPONENTS OF THE EPITHELIAL CELLS OF RABBIT SMALL INTESTINE.

Authors:  J W PORTEOUS; B CLARK
Journal:  Biochem J       Date:  1965-07       Impact factor: 3.857
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  59 in total

1.  Purification and characterization of a phosphatidylinositol 3-kinase complex from bovine brain by using phosphopeptide affinity columns.

Authors:  M J Fry; G Panayotou; R Dhand; F Ruiz-Larrea; I Gout; O Nguyen; S A Courtneidge; M D Waterfield
Journal:  Biochem J       Date:  1992-12-01       Impact factor: 3.857

2.  Purification and characterization of a higher-molecular-mass form of protein phosphotyrosine phosphatase (PTP 1B) from placental membranes.

Authors:  C J Pallen; D S Lai; H P Chia; I Boulet; P H Tong
Journal:  Biochem J       Date:  1991-06-01       Impact factor: 3.857

3.  Blood platelets express high levels of the pp60c-src-specific tyrosine kinase activity.

Authors:  A Golden; S P Nemeth; J S Brugge
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

4.  Superagonistic behaviour of epidermal growth factor/transforming growth factor-alpha chimaeras: correlation with receptor routing after ligand-induced internalization.

Authors:  A E Lenferink; R H Kramer; M J van Vugt; M Königswieser; P P Di Fiore; E J van Zoelen; M L van de Poll
Journal:  Biochem J       Date:  1997-11-01       Impact factor: 3.857

5.  Protein kinase activity associated with the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10).

Authors:  T D Chung; J P Wymer; C C Smith; M Kulka; L Aurelian
Journal:  J Virol       Date:  1989-08       Impact factor: 5.103

6.  Specific dephosphorylation of phosphoproteins by protein-serine and -tyrosine kinases.

Authors:  H K Kole; M Abdel-Ghany; E Racker
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

7.  Binding of lipopolysaccharide (LPS) to an 80-kilodalton membrane protein of human cells is mediated by soluble CD14 and LPS-binding protein.

Authors:  J Schletter; H Brade; L Brade; C Krüger; H Loppnow; S Kusumoto; E T Rietschel; H D Flad; A J Ulmer
Journal:  Infect Immun       Date:  1995-07       Impact factor: 3.441

8.  Physicochemical characterization of the cytoplasmic domain of the epidermal growth factor receptor and evidence for conformational changes associated with its activation by ammonium sulphate.

Authors:  M Gregoriou; P F Jones; J F Timms; J J Yang; S E Radford; A R Rees
Journal:  Biochem J       Date:  1995-03-15       Impact factor: 3.857

9.  Phorbol esters potentiate tyrosine phosphorylation of epidermal growth factor receptors in A431 membranes by a calcium-independent mechanism.

Authors:  S O Moon; H C Palfrey; A C King
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

10.  The Fas protein is expressed at high levels on CD4+CD8+ thymocytes and activated mature lymphocytes in normal mice but not in the lupus-prone strain, MRL lpr/lpr.

Authors:  J Drappa; N Brot; K B Elkon
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-01       Impact factor: 11.205
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