Literature DB >> 14343125

THE ISOLATION AND CHARACTERIZATION OF SUBCELLULAR COMPONENTS OF THE EPITHELIAL CELLS OF RABBIT SMALL INTESTINE.

J W PORTEOUS, B CLARK.   

Abstract

1. Homogenization of the epithelial cells of rabbit small intestine in 0.3m-sucrose-5mm-EDTA, pH7.4, maintains intact the microvillus sheets that form the lumenal surface of the cells, the nuclei, the mitochondria and the vesicles (microsomes) formed from the endoplasmic reticulum. 2. These particulate components of the cell, and the cell-sap fraction, have been isolated by differential centrifuging of cell homogenates. 3. The nuclei and microvillus sheets sediment together and it has been impossible to separate these subcellular components by centrifugal methods. 4. The isolated subcellular fractions have been identified by a combination of light-microscopic examination, electron-microscopic examination, chemical analysis and assay for selected enzyme activities.

Entities:  

Keywords:  ACID PHOSPHATASE; ALKALINE PHOSPHATASE; AMINOPEPTIDASES; BIOCHEMISTRY; CENTRIFUGATION; CHOLESTEROL; DNA; EPITHELIUM; EXPERIMENTAL LAB STUDY; GLUCOSEPHOSPHATASE; GLYCOSIDE HYDROLASES; HOMOGENATES; INTESTINAL MUCOSA; INTESTINE, SMALL; MICROSCOPY, ELECTRON; RABBITS; RNA; SUCCINATE DEHYDROGENASE

Mesh：

Substances：

Year:  1965        PMID： 14343125      PMCID： PMC1206917          DOI： 10.1042/bj0960159

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  46 in total

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Authors:  J M BAILEY; P PENTCHEV
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2.  LOCATION OF HYDROLYSIS OF CERTAIN DISACCHARIDES AND PEPTIDES IN THE SMALL INTESTINE.

Authors:  A M UGOLEV; N N IESUITOVA; N M TIMOFEEVA; I N FEDIUSHINA
Journal:  Nature       Date:  1964-05-23       Impact factor: 49.962

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Journal:  Nature       Date:  1964-10-10       Impact factor: 49.962

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Journal:  Biochem J       Date:  1932       Impact factor: 3.857

5.  Histological Localization of Newly-formed Desoxyribonucleic Acid.

Authors:  C P Leblond; C E Stevens; R Bogoroch
Journal:  Science       Date:  1948-11-12       Impact factor: 47.728

6.  The digestive function of the epithelium of the small intestine. I. An intracellular locus of disaccharide and sugar phosphate ester hydrolysis.

Authors:  D MILLER; R K CRANE
Journal:  Biochim Biophys Acta       Date:  1961-09-16

7.  Glucose absorption from surviving rat small intestine.

Authors:  R B FISHER; D S PARSONS
Journal:  J Physiol       Date:  1949-12       Impact factor: 5.182

8.  THE METAL ION ACTIVATION OF THE ALKALINE BETA-GLYCEROPHOSPHATASE OF RABBIT SMALL INTESTINE.

Authors:  B CLARK; J W PORTEOUS
Journal:  Biochem J       Date:  1965-05       Impact factor: 3.857

9.  A study of fixation for electron microscopy.

Authors:  G E PALADE
Journal:  J Exp Med       Date:  1952-03       Impact factor: 14.307

10.  STUDIES ON SMALL INTESTINAL CRYPT EPITHELIUM. I. THE FINE STRUCTURE OF THE CRYPT EPITHELIUM OF THE PROXIMAL SMALL INTESTINE OF FASTING HUMANS.

Authors:  J S TRIER
Journal:  J Cell Biol       Date:  1963-09       Impact factor: 10.539
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  52 in total

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Authors:  B A Lewis; A Elkin; R H Michell; R Coleman
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Journal:  Cell Tissue Res       Date:  1992-12       Impact factor: 5.249

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Authors:  J W Porteous; H M Furneaux; C K Pearson; C M Lake; A Morrison
Journal:  Biochem J       Date:  1979-06-15       Impact factor: 3.857

4.  Rabbit distal colon epithelium: I. Isolation and characterization of basolateral plasma membrane vesicles from surface and crypt cells.

Authors:  H Wiener; K Turnheim; C H van Os
Journal:  J Membr Biol       Date:  1989-09       Impact factor: 1.843

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Authors:  J L Harwood; P K Stumpf
Journal:  Plant Physiol       Date:  1970-10       Impact factor: 8.340

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Authors:  K A Lloyd-Davies; R H Michell; R Coleman
Journal:  Biochem J       Date:  1972-04       Impact factor: 3.857

7.  The relationship of detergent-solubilization plasma-membrane components of rabbit alveolar macrophages to an isolated inhibitor of phagocytosis.

Authors:  R S Pratt; G M Cook
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8.  The absorption of protons with alpha-methyl glucoside and alpha-thioethyl glucoside by the yeast N.C.Y.C. 240. Evidence against the phosphorylation hypothesis.

Authors:  R Brocklehurst; D Gardner; A A Eddy
Journal:  Biochem J       Date:  1977-03-15       Impact factor: 3.857

9.  Functional changes in digestive enzyme activities of meagre (Argyrosomus regius; Asso, 1801) during early ontogeny.

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10.  Long-chain fatty Acyl-CoA synthetase enzymatic activity in rat liver cell nuclei.

Authors:  A Ves-Losada; R R Brenner
Journal:  Mol Cell Biochem       Date:  1996-06-07       Impact factor: 3.396
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